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ZFIN ID: ZDB-FIG-151214-3
Kuan et al., 2015 - Distinct requirements for Wntless in habenular development. Developmental Biology   406(2):117-28 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Day 4
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-15 to Day 4

Fig. 3

Wnt signaling is reduced in wls mutants. (A–H) The c186 mutation was introduced into a transgenic reporter of canonical Wnt signaling, Tg(7xTCF-Xla.Siam:GFP)ia4 (Moro et al., 2012). At 30 hpf, (A,B) WT siblings show robust GFP labeling in the dorsal diencephalon, midbrain–hindbrain boundary and otic vesicles compared to (E,F) c186 mutants (A, E lateral views, B, F frontal views). By 60 hpf, GFP labeling is also found in the pectoral fins and jaw of (C, D) WT embryos and is reduced in (G,H) c186 mutants (C, G lateral views, D, H dorsal views). (I,J) GFP labeling from Tg(7xTCF-Xla.Siam:GFP)ia4 colocalizes with dbx1b expression in the presumptive habenulae at 27 hpf (single section) and 35 hpf (maximum projection of 15 sections at 0.3 µm) (K,M) WT and (L,N) c186 mutant embryos were assayed for expression of the Wnt-responsive genes (K,L) lef1 and (M,N) axin2. Bilateral lef1 expression domains in the dorsal diencephalon (arrows in K) are absent in wls mutants at 4 dpf, as are axin2 transcripts in a similar region of the brain (arrow in M). Expression of axin2 is also reduced in the developing jaw and otic vesicles of (N) wls mutants. (K,L dorsal, M,N lateral views). Scale bar=100 µm for A–H and K–N and 50 µm for I,J.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
axin2 AB standard conditions Day 4 diencephalon dorsal region ISH
Day 4 mandibular arch skeleton ISH
Day 4 otic vesicle ISH
wlsc186/c186(AB) standard conditions Day 4 diencephalon dorsal region ISH
Day 4 mandibular arch skeleton ISH
Day 4 otic vesicle ISH
dbx1b ia4Tg standard conditions Prim-5 epithalamus ISH
Prim-15 epithalamus ISH
GFP ia4Tg standard conditions Prim-5 epithalamus IHC
Prim-15 diencephalon dorsal region IFL
Prim-15 epithalamus IHC
Prim-15 midbrain hindbrain boundary IFL
Prim-15 otic vesicle IFL
Pec-fin diencephalon dorsal region IFL
Pec-fin mandibular arch skeleton IFL
Pec-fin midbrain hindbrain boundary IFL
Pec-fin otic vesicle IFL
Pec-fin pectoral fin IFL
wlsc186/c186 ; ia4Tg standard conditions Prim-15 diencephalon dorsal region IFL
Prim-15 midbrain hindbrain boundary IFL
Prim-15 otic vesicle IFL
Pec-fin diencephalon dorsal region IFL
Pec-fin mandibular arch skeleton IFL
Pec-fin midbrain hindbrain boundary IFL
Pec-fin otic vesicle IFL
Pec-fin pectoral fin IFL
lef1 AB standard conditions Day 4 diencephalon dorsal region ISH
Day 4 midbrain ISH
wlsc186/c186(AB) standard conditions Day 4 diencephalon dorsal region ISH
Day 4 midbrain ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
wlsc186/c186(AB) standard conditions Day 4 diencephalon dorsal region axin2 expression decreased amount, abnormal
Day 4 diencephalon dorsal region lef1 expression decreased amount, abnormal
Day 4 mandibular arch skeleton axin2 expression decreased amount, abnormal
Day 4 otic vesicle axin2 expression decreased amount, abnormal
wlsc186/c186 ; ia4Tg standard conditions Prim-15 diencephalon dorsal region GFP expression decreased amount, abnormal
Prim-15 midbrain hindbrain boundary GFP expression decreased amount, abnormal
Prim-15 otic vesicle GFP expression decreased amount, abnormal
Pec-fin diencephalon dorsal region GFP expression decreased amount, abnormal
Pec-fin mandibular arch skeleton GFP expression decreased amount, abnormal
Pec-fin midbrain hindbrain boundary GFP expression decreased amount, abnormal
Pec-fin otic vesicle GFP expression decreased amount, abnormal
Pec-fin pectoral fin GFP expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 406(2), Kuan, Y.S., Roberson, S., Akitake, C.M., Fortuno, L., Gamse, J., Moens, C., Halpern, M.E., Distinct requirements for Wntless in habenular development, 117-28, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.