Fig. S2
- ID
- ZDB-FIG-151120-27
- Publication
- Dzafic et al., 2015 - Centriole Amplification in Zebrafish Affects Proliferation and Survival but Not Differentiation of Neural Progenitor Cells
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Related to Figure 1. (A) Centriole number quantification in interphase neuroepithelial cells in the WT and Tg(OS-Plk4) fish based on the EM data. HS applied at 24hpf and fish were fixed at 10hphs. (B) Centriole number quantification in mitotic neuroepithelial cells in the WT and Tg(OS-Plk4) fish obtained from en face imaging of centrin stained samples. HS applied at 24hpf and fish were fixed at 16hphs. (C) Summary of centriole number abnormalities observed in Tg(OS-Plk4) mitotic cells, fixed at 16hphs and imaged en face following centrin antibody staining. Percentage of total number of cells counted for each category as well as in the overlap between categories is shown. (D) Graphs showing the spread of centriole numbers within single embryos obtained from EM analysis (top) and IF analysis (bottom). (E) Confocal scans of retinae of WT fish injected with HS-OS-Plk4 at different timepoints after HS applied at 24hpf, stained for active-caspase-3 (apoptotic marker, green) and DAPI (grey). Scale bar is 50?m. (F) Confocal scans of retinae of WT control fish at different timepoints after HS applied at 24hpf, stained for active-caspase-3 (apoptotic marker, green) and DAPI (grey). Scale bar is 50?m. |