Fig. 6
- ID
- ZDB-FIG-151120-24
- Publication
- Dzafic et al., 2015 - Centriole Amplification in Zebrafish Affects Proliferation and Survival but Not Differentiation of Neural Progenitor Cells
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Centriole Amplification Affects the Proliferative Phase of Retinal Development but Not Neuronal Differentiation (A?C) Confocal scans of the retinae of control (A) and transgenic Tg(HS-OS-Plk4) fish (B and C) showing milder (B) or severe (C) phenotypes; HS was applied at 24 hpf, and these embryos were fixed at 80 hpf and stained for Zn-5 (retinal ganglion cells, or RGCs; magenta) and DAPI (nuclei, green/gray). Arrows label holes in nuclear arrangement. In fish with milder phenotype (B), some neuronal layering can be observed corresponding to RGC layer and photoreceptor cell (PRC) layer. (D) Confocal scan of the retina of a Tg(HS-OS-Plk4) fish; HS was applied at 16 hpf, and these embryos were fixed at 80 hpf and stained for Zn-5 (magenta) and DAPI (green/gray). Arrows label large holes in the tissue. (E) Confocal scan of the retina of Tg(HS-OS-Plk4) fish crossed to Tg(Ath5-gapRFP) (marker of early born neurons: RGCs and PRCs, green) and Tg(Ptf1A-GFP) (marker of later born neurons: amacrine and horizontal cells, magenta) line; HS was applied at 24 hpf, and these embryos were fixed at 76 hpf and stained for DAPI. (F) Confocal scan of the retina of Tg(HS-OS-Plk4) fish crossed to Tg(Vsx1-GFP) (marker of late-born bipolar cells, green) and Tg(Ptf1A-dsRed) (magenta) lines, HS was applied at 24 hpf, and these embryos were fixed at 96 hpf and stained for DAPI. (G) Confocal scan of the retina of fish injected with HS-OS-Plk4 construct and HS-H2B-GFP construct (labeling OS-Plk4-expressing cells, green); HS was applied at 24 hpf, and these embryos were fixed at 80 hpf and stained for HuC/D (RGCs and amacrine cell marker, magenta) and DAPI (blue). (H) Confocal scans of retinae from crosses of Tg(HS-OS-Plk4) fish to Tg(Ath5-gapRFP) (green) and Tg(Ptf1A-GFP) (magenta) lines (right panel) and Tg(Ath5-gapRFP) (green)/Tg(Ptf1A-GFP) (magenta) line only (control, left panel); HS was applied at 24 hpf, and these embryos were fixed at 50 hpf and stained for DAPI (blue). (I and J) Confocal scan of the retina of Tg(HS-OS-Plk4) fish crossed to the Tg(Ath5-gapRFP) (green)/Tg(Ptf1A-GFP) (magenta) line (I) or Tg(Vsx1-GFP) (green)/Tg(Ptf1A-dsRed) (magenta) line (J). HS was applied at 48 hpf, and these embryos were fixed at 78 hpf and stained for DAPI (gray/blue). Arrows point at smaller holes/apoptotic regions in the tissue. Scale bars, 50 µm. |