ZFIN ID: ZDB-FIG-151113-9
Hoeppner et al., 2015 - RhoC maintains vascular homeostasis by regulating VEGF-induced signaling in endothelial cells. Journal of Cell Science   128(19):3556-68 Full text @ J. Cell Sci.
Anatomical Terms:
Stage: Prim-5

Fig. 7

RhoC negatively regulates VEGF-induced vascular permeability in zebrafish. (A,B) Using zebrafish cDNA, a probe to zebrafish RhoC (Rhoad) was created and in situ hybridization was performed on 24hpf zebrafish embryos. Multiple images were captured using a Zeiss Axioplan 2 microscope and overlayed in Photoshop, such that areas of focus were unmasked, to generate a composite image. Lateral (A) and superior (B) views are shown. DA, dorsal aorta; PCV, posterior cardinal vein; ISVs, anterior intersomitic vessels; NT, neural tubes. (C) Microangiography was performed on anesthetized 3dpf zebrafish embryos by injecting FITC–dextran (2000kDa) and Texas-Red–dextran (70kDa), VEGF was induced through heat exposure (when applicable), and extravasation of red tracer as a measure of zebrafish vascular permeability was live imaged using a ZEISS LSM 780 confocal microscope. Control, no MO injection and no VEGF induction; Cont MO, control MO injection; RhoC MO, Rhoad and Rhoae MO injection; Unind, no VEGF induction; VEGF, heat induction of VEGF transgene. (D) Quantification of extravasated red tracer. **P<0.05 (RhoC MO, VEGF Induced versus Control MO, VEGF Induced); *P<0.05 (RhoC MO, Uninduced versus Control; Control MO, VEGF Induced versus Control) (paired two-tailed Student′s t-test). Results are mean±s.d. (n=??).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
rhocb WT standard conditions Prim-5 dorsal aorta ISH
Prim-5 intersegmental vessel ISH
Prim-5 neural tube ISH
Prim-5 posterior cardinal vein ISH
Antibody Labeling Details No data available
Phenotype Details No data available
ZFIN wishes to thank the journal Journal of Cell Science for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ J. Cell Sci.