FIGURE

Fig. 5

ID

ZDB-FIG-151112-33

Publication

Yao et al., 2015 - Differential regulation of Kit Ligand A (kitlga) expression in the zebrafish ovarian follicle cells - evidence for the existence of a cyclic adenosine 3', 5' monophosphate-mediated binary regulatory system during folliculogenesis

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Fig. 5

Effects of IGF-I on the expression of kitlga in the presence of db-cAMP (A), forskolin (B), and H89 (C). Cultured zebrafish follicle cells were pretreated with db-cAMP (1.5?mM), forskolin (10?�M), or H89 (10?�M) for 30?min before treatment with IGF-I (50?ng/mL) for 6?h. (D) Interactive effects of the PKA and PI3K-Akt pathways on kitlga expression. Cultured zebrafish follicle cells were treated with H89 (10?�M) and LY294002 (50?�M) alone or in combination for 6?h. The data were normalized to the housekeeping gene ef1a and expressed as a percentage of the control group (mean?�?SEM, n?=?4). Different letters indicate statistical significance (P?

Expression Data

Gene:	kitlga
Fish:	WT
Conditions:	chemical treatment: bucladesine, primary cell culture: ovarian follicle chemical treatment: bucladesine, primary cell culture: ovarian follicle chemical treatment: forskolin, primary cell culture: ovarian follicle chemical treatment: Insulin-like growth factor I, primary cell culture: ovarian follicle chemical treatment: Insulin-like growth factor I, primary cell culture: ovarian follicle
Anatomical Term:	ovarian follicle
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Molecular and Cellular Endocrinology, 402, Yao, K., Ge, W., Differential regulation of Kit Ligand A (kitlga) expression in the zebrafish ovarian follicle cells - evidence for the existence of a cyclic adenosine 3', 5' monophosphate-mediated binary regulatory system during folliculogenesis, 21-31, Copyright (2015) with permission from Elsevier. Full text @ Mol. Cell. Endocrinol.