Fig. 3

slc26a2 knockdown in wild-type embryos and rescue of slc26a2 overexpression in S2-SP1 morphants.

(A) Variations in otolith size of morphant (S2-SP1 knockdown) embryos were observed at 72hpf. Based on the otolith size, malformed embryos were classified as type A (abnormal phenotype, including small, tiny, fused and misplaced otoliths) or type B (abnormal number of otoliths, including increased and decreased otoliths). (B) Morphology of 72hpf WT embryos injected with different morpholinos. One or two cell embryos were injected with 4 ng of S2-SP1 or Mcon. WT. Note the change in otolith size. (C) Schematic representation of two functional domains of zebrafish and human slc26a2 proteins. Degree of identity/similarity indicated for the sulphate transporter, STAS, and the C terminal dimerization domains. (D) Relative numbers of embryos in each category. Single-celled embryos were injected with indicated morpholinos at the indicated dose and categorized at 72hpf. Single-celled S2-SP1 embryos were either uninjected or were injected with 150 pg of human SLC26A2 mRNA and categorized at 72 hpf. (N = number of observed embryos).