FIGURE

Fig. 5

ID

ZDB-FIG-151029-25

Publication

Chen et al., 2015 - Transient laminin beta 1a Induction Defines the Wound Epidermis during Zebrafish Fin Regeneration

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Fig. 5

lamb1a is required for juvenile fin growth but not body growth.

(A) Juvenile sde1 animals, after incubation from 4 to 6 weeks post-fertilization (wpf) at 33°C, acquire degraded fins. Scale bars, 2 mm. (B) sde1 mutations have minimal impact on juvenile outgrowth. Body length was measured from the tip of the snout to the base of caudal fin. (C) RT-qPCR analysis indicates that both lamb1a and lamb1b are induced in fin tissue during juvenile outgrowth. qPCR results were normalized to rpl13a and to the basal expression of lamb1a/lamb1b in adult uninjured fins (n = 4; mean ± SEM). (D) Antibody staining for Laminin expression in juvenile fins. Laminin protein in longitudinal sections of fins is localized to the basal side of the epitheilum in sde1/+ animals, but becomes mislocalized in sde1 mutants.

Expression Data

Genes:	lamb1a lamb1b
Antibody:	Ab1-lama1
Fish:	WT lamb1a^pd110/pd110 lamb1a^pd110/+
Condition:	amputation: caudal fin, heat exposure
Anatomical Terms:	fin fin epithelium
Stage Range:	Days 30-44 to Days 45-89

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	lamb1a^pd110/pd110
Conditions:	heat exposure amputation: caudal fin, heat exposure
Observed In:	fin fin epithelium
Stage Range:	Days 21-29 to Days 30-44

Phenotype Detail

Acknowledgments

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