FIGURE

Fig. 8

ID

ZDB-FIG-150812-4

Publication

Giacomotto et al., 2015 - Effective heritable gene knockdown in zebrafish using synthetic microRNAs

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Fig. 8

hsa-SMN1 rescues the dre-smn1 knockdown motor neuron phenotype.

Representative images of 52hpf zebrafish larvae expressing GFP in motor neurons. Lateral views of (a) control (empty vector) transgenic animals. (b–d) transgenic UBI:miRsmn1-1#5 zebrafish line (presenting ~90% downregulation of SMN), with hsa-SMN1 RNA injection (c) or hsa-SMN1 ubiquitous expression via integrated transgene (d; see Supplementary Fig. 7 for strategy used). Scale bar, 50µm.

Expression Data

Gene:	GFP
Fish:	bi124Tg bi124Tg; zf2041Tg bi124Tg; zf2040Tg; zf2041Tg
Anatomical Term:	motor neuron
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	bi124Tg; zf2041Tg bi124Tg; zf2040Tg; zf2041Tg
Observed In:	CaP motoneuron CaP motoneuron axon CaP motoneuron axon guidance
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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