FIGURE

Fig. 5

ID

ZDB-FIG-150812-3

Publication

Giacomotto et al., 2015 - Effective heritable gene knockdown in zebrafish using synthetic microRNAs

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Fig. 5

Representative images of 52 hpf zebrafish larvae expressing GFP in motor neurons ± smn1 knockdown.

Lateral views of (a) control (empty vector) and (b-e) transgenic zebrafish larvae expressing artificial miR (targeting smn1 3′-UTR). Compared with control animals, transgenic larvae expressing miRsmn1 present abnormal motor neuron development, including abnormal branching, short axons as well as pathfinding errors (white arrowheads). Transgenic animals expressing artificial miRNA against the ORF (targets 2 and 3) do not show obvious motor neuron abnormality. Scale bar, 50µm.

Expression Data

Gene:	GFP
Fish:	bi124Tg bi124Tg; zf2041Tg bi124Tg; zf2042Tg
Anatomical Term:	motor neuron
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	bi124Tg; zf2041Tg bi124Tg; zf2042Tg
Observed In:	CaP motoneuron CaP motoneuron axon CaP motoneuron axon guidance
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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