Fig. 3
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- ZDB-FIG-150805-9
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- Compagnon et al., 2014 - The Notochord Breaks Bilateral Symmetry by Controlling Cell Shapes in the Zebrafish Laterality Organ
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The Notochord Affects KV Cell Shape by Polarizing Laminin Deposition around KV (A) α-Laminin-α1β1γ1 antibody staining of KV in Tg(sox17:GFP) embryos at 1 ss (10.3 hpf; coronal section, A.1) and 6 ss (12 hpf; sagittal section, A.2 and A.3); A.4 and A.5 show insets of the region boxed in upper panels. (B) Thickness of the KV epithelium [n = 42 measurements on 3 Tg(sox17:GFP) embryos]; box and whisker plot. (C) Basal α-laminin-α1β1γ1 antibody staining intensity along KV epithelium in the same sections used for (B); n = 6 lines measurements; see dashed line in (A) for location; box and whisker plot; red line, trend based on the local average method. (D) α-FN-1 antibody and DAPI staining on 6 ss (12 hpf) Tg(sox17:GFP) embryo (coronal section). (E) α-Laminin-α1β1γ1 antibody and DAPI staining on ectopic KV in 6 ss (12 hpf) Tg(sox17:GFP) (left panel), MZoep; Tg(sox17:GFP) (middle panel), and MZoep; Tg(sox17:GFP) with a coinduced notochord (arrow in right panel) embryos. (F) Ratio of nuclei densities between the AD and PV quadrants of KV as a function of average KV cell apical surface; mean ± SEM. Control n = 11 embryos; laminin γ1-MO1-cell n = 15 embryos; laminin γ1-MODFC n = 7 embryos; embryos injected with 40 pg truncated FN 1a and 1b mRNA n = 4 embryos. Data were polled from Tg(sox17:GFP) and Tg(sox17:LMA-tdTomato) embryos between 1 ss and 10 ss (10.3-14 hpf). (G) Projected cilia length in control embryos and embryos injected with laminin γ1 MO either into the YSL between the 512- and 1,000-cell stages or at the 1-cell stage. p < 0.0001 (t test); n > 140 cilia for each condition; n > 5 embryos for each condition. (H) Projected cilia length in control embryos and embryos injected with 40 pg truncated FN 1a and 1b mRNA; n > 89 cilia for each condition; n > 3 embryos for each condition; ns, p value (t test) > 0.05 (I) Quantification of the lateralization of spaw expression between 18 ss and 22 ss (20–22 hpf) by in situ hybridization in control (n = 18 embryos) and embryos injected with 40 pg truncated FN 1a and 1b mRNA (n = 36 embryos). A, anterior; P, posterior; L, left; R, right; V, ventral; D, dorsal; ns, not significant; YSL, yolk syncytial layer; ctrl, control; trunc. fibro., embryos injected with 40 pg truncated FN 1a and 1b mRNA. Scale bars in (A), (D), and (E), 20 µm. See also Figure S3. |
Reprinted from Developmental Cell, 31, Compagnon, J., Barone, V., Rajshekar, S., Kottmeier, R., Pranjic-Ferscha, K., Behrndt, M., Heisenberg, C., The Notochord Breaks Bilateral Symmetry by Controlling Cell Shapes in the Zebrafish Laterality Organ, 774-783, Copyright (2014) with permission from Elsevier. Full text @ Dev. Cell