PUBLICATION

The Notochord Breaks Bilateral Symmetry by Controlling Cell Shapes in the Zebrafish Laterality Organ

Authors
Compagnon, J., Barone, V., Rajshekar, S., Kottmeier, R., Pranjic-Ferscha, K., Behrndt, M., Heisenberg, C.
ID
ZDB-PUB-141224-8
Date
2014
Source
Developmental Cell   31: 774-783 (Journal)
Registered Authors
Barone, Vanessa, Behrndt, Martin, Compagnon, Julien, Heisenberg, Carl-Philipp, Rajshekar, Shrivarsha
Keywords
none
MeSH Terms
  • Cilia/physiology
  • Epithelial Cells/cytology
  • Extracellular Matrix/metabolism
  • Cell Shape*
  • Zebrafish/embryology*
  • Body Patterning*
  • Stem Cells/cytology
  • Zebrafish Proteins/metabolism
  • Gene Expression Regulation, Developmental
  • Animals
  • Cell Nucleus/metabolism
  • Somites/metabolism
  • Epithelium/metabolism
  • Notochord/embryology*
  • Notochord/metabolism
(all 15)
PubMed
25535919 Full text @ Dev. Cell
Abstract
Kupffer's vesicle (KV) is the zebrafish organ of laterality, patterning the embryo along its left-right (LR) axis. Regional differences in cell shape within the lumen-lining KV epithelium are essential for its LR patterning function. However, the processes by which KV cells acquire their characteristic shapes are largely unknown. Here, we show that the notochord induces regional differences in cell shape within KV by triggering extracellular matrix (ECM) accumulation adjacent to anterior-dorsal (AD) regions of KV. This localized ECM deposition restricts apical expansion of lumen-lining epithelial cells in AD regions of KV during lumen growth. Our study provides mechanistic insight into the processes by which KV translates global embryonic patterning into regional cell shape differences required for its LR symmetry-breaking function.
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Citation
Compagnon, J., Barone, V., Rajshekar, S., Kottmeier, R., Pranjic-Ferscha, K., Behrndt, M., Heisenberg, C. (2014) The Notochord Breaks Bilateral Symmetry by Controlling Cell Shapes in the Zebrafish Laterality Organ. Developmental Cell. 31:774-783.