FIGURE

Fig. 3

ID

ZDB-FIG-150805-3

Publication

Labalette et al., 2015 - Molecular dissection of segment formation in the developing hindbrain

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Fig. 3

Nlz factors mediate Hox PG1 repression of krox20. (A-D) Wild-type (WT) embryos were co-injected with 50ng/µl of hoxb1a or cherry-h2b (control) mRNAs, together with either a control morpholino (moCtrl) or morpholinos for nlz1 and nlz2 (moNlz). Embryos were collected at 5s and subjected to in situ hybridization with a krox20 probe. (E-H) WT or Tg(hsp:hoxb1a) embryos were injected with either moCtrl or moNlz, heat-shocked for 30min at 100% epiboly, collected at 20s and subjected to ISH with krox20 (purple) and pax2.1+val (orange) probes. mhb, mid-hindbrain boundary.

Expression Data

Gene:	egr2b
Fish:	WT zf1076Tg WT + MO3-znf503 + MO3-znf703 + MO4-znf503 + MO4-znf703 zf1076Tg + MO3-znf503 + MO3-znf703 + MO4-znf503 + MO4-znf703
Condition:	heat shock
Knockdown Reagents:	MO3-znf503 MO3-znf703 MO4-znf503 MO4-znf703
Anatomical Terms:	rhombomere 2 rhombomere 3 rhombomere 4 rhombomere 5
Stage:	20-25 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	zf1076Tg WT + MO3-znf503 + MO3-znf703 + MO4-znf503 + MO4-znf703 zf1076Tg + MO3-znf503 + MO3-znf703 + MO4-znf503 + MO4-znf703
Condition:	heat shock
Knockdown Reagents:	MO3-znf503 MO3-znf703 MO4-znf503 MO4-znf703
Observed In:	rhombomere 2 rhombomere 3 rhombomere 4 rhombomere 5
Stage:	20-25 somites

Phenotype Detail

Acknowledgments

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