FIGURE

Fig. 1

ID

ZDB-FIG-150518-1

Publication

Oltrabella et al., 2015 - The Lowe Syndrome Protein OCRL1 Is Required for Endocytosis in the Zebrafish Pronephric Tubule

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Fig. 1

Impairment of pronephric uptake in OCRL1 deficient zebrafish embryos.

A. Confocal images of wild-type (WT), ocrl^-/- mutant, control morphant or OCRL1 morphant 72 hpf zebrafish embryos that were injected with Alexa 488-10 kDa dextran (white) and imaged after 2.5 h. The pronephric tubules are indicated with a green dashed line. B. Top: Quantification of pronephric uptake of 10 kDa (2.5 h) or 70 kDa dextran (4 h) in control, ocrl^-/- mutant and morphant embryos. Bottom: Representation of normal, low and no dextran uptake in injected. C. Wild-type (WT) and ocrl^-/- mutant embryos were injected with RAP-Cy3 (red) and pronephric accumulation after 60 min monitored by fluorescence microscopy. D. Quantification of pronephric uptake of RAP-Cy3 in control and ocrl^-/- mutant embryos. Data are presented as the mean ± SD. Statistical analysis was performed using the Pearson’s chi-squared test. ***p < 0.0001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ocrl^{umc5Tg/umc5Tg} AB + MO1-ocrl
Knockdown Reagent:	MO1-ocrl
Observed In:	pronephros renal absorption
Stage:	Day 5

Phenotype Detail

Acknowledgments

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