ZFIN Image: Oltrabella et al., 2015, Fig. 1

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Fig. 1

Impairment of pronephric uptake in OCRL1 deficient zebrafish embryos.

A. Confocal images of wild-type (WT), ocrl^-/- mutant, control morphant or OCRL1 morphant 72 hpf zebrafish embryos that were injected with Alexa 488-10 kDa dextran (white) and imaged after 2.5 h. The pronephric tubules are indicated with a green dashed line. B. Top: Quantification of pronephric uptake of 10 kDa (2.5 h) or 70 kDa dextran (4 h) in control, ocrl^-/- mutant and morphant embryos. Bottom: Representation of normal, low and no dextran uptake in injected. C. Wild-type (WT) and ocrl^-/- mutant embryos were injected with RAP-Cy3 (red) and pronephric accumulation after 60 min monitored by fluorescence microscopy. D. Quantification of pronephric uptake of RAP-Cy3 in control and ocrl^-/- mutant embryos. Data are presented as the mean ± SD. Statistical analysis was performed using the Pearson’s chi-squared test. ***p < 0.0001.

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Acknowledgments

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