ZFIN ID: ZDB-FIG-150504-32
Quan et al., 2015 - Comparative Distribution and In Vitro Activities of the Urotensin II-Related Peptides URP1 and URP2 in Zebrafish: Evidence for Their Colocalization in Spinal Cerebrospinal Fluid-Contacting Neurons. PLoS One   10:e0119290 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
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Anatomical Term:
Stage: Adult

Fig. 7 Both urp1+ and urp2+ cells in the spinal cord are GABAergic neurons in adult zebrafish.

Simultaneous expression of urp1 and urp2 revealed by double fluorescent ISH (TAMRA, red for urp1 and Cy5, white for urp2) in adult spinal cord, coupled to a fluorescent immunostaining for GAD65/67 (Alexa Fluor 488, green) (A, B). Both urp1+ and urp2+ cells are GAD+ (A, B). Arrows designate triple-stained cells (A, B). Note the occurrence of some doubly-positive cells (urp2/GAD) that do not contain any urp1 (arrowhead) (A). Asterisks designate GABAergic interneurons located at the dorsal part of the spinal cord. The white dash line indicates the central canal. A, sagittal section with dorsal up; B, coronal section with dorsal up. Scale bars: 15 ┬Ám.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
urp1 WT standard conditions Adult spinal cord GABAergic neuron ISH
urp2 WT standard conditions Adult spinal cord GABAergic neuron ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-gad IHC WT standard conditions Adult spinal cord GABAergic neuron
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One