Fig. 2
- ID
- ZDB-FIG-150423-8
- Publication
- Tessadori et al., 2015 - Nodal Signaling Range Is Regulated by Proprotein Convertase-Mediated Maturation
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FurinA Is Required Cell-Autonomously to Process Spaw and to Confer Its Long-Range Signaling Activity (A) Schematic of the transplantation procedure. (B and C) Both MZspaw-/- (B) and MZaoh-/- (C) embryos fail to express spaw in the left LPM at 18 somites. (D) ISH for spaw (blue staining) with immunolabeling for transplanted cells (red staining). Transplantation of spaw-injected spaw-/- cells (yellow clone, red outline) in MZspaw-/- resulted in the rescue of spaw expression in the left LPM several cell diameters away from the transplanted clone. (E) Transplantation of cells injected with RNA of a noncleavable form of spaw (yellow clone, purple outline) in MZspaw-/- failed to induce of spaw expression in the left LPM. (F) Transplantation of spaw-injected spaw-/- cells (yellow clone, red outline) in MZaoh-/- resulted in induction of spaw expression in the left LPM several cell diameters away from the transplanted clone. (G) Spaw-injected aoh-/- cells (green clone, red outline) in MZspaw-/- were unable to induce spaw expression in the left LPM. In all schematics, MZspaw-/- cells appear in yellow and MZaoh-/- cells in green. |
| Gene: | |
|---|---|
| Fish: | |
| Stage: | 14-19 somites |
Reprinted from Developmental Cell, 32(5), Tessadori, F., Noël, E.S., Rens, E.G., Magliozzi, R., Evers-van Gogh, I.J., Guardavaccaro, D., Merks, R.M., Bakkers, J., Nodal Signaling Range Is Regulated by Proprotein Convertase-Mediated Maturation, 631-9, Copyright (2015) with permission from Elsevier. Full text @ Dev. Cell