FIGURE
Fig. S5
Fig. S5
miR-9 overexpression strategy and stabilization of dicer, miR-9 and ngn1 hindbrain expression upon dicer-3′UTR protection. Related to Figure 7. (A) Cartoon describing the strategy adopted for the inducible miR-9 gain-of-function experiment (see Experimental Procedures for details). (B) Fluorescent and bright-field images of whole-mount zebrafish embryos at 48 hpf injected with the Hsp70-miR-9mCherry plasmid at the one-cell stage and heatshocked at 35 hpf for 15 min at 42°C. Anterior is to the left. |
Expression Data
| Gene: | |
|---|---|
| Fish: | |
| Knockdown Reagents: | |
| Anatomical Term: | |
| Stage: | Pec-fin |
Expression Detail
Antibody Labeling
Phenotype Data
| Fish: | |
|---|---|
| Knockdown Reagents: | |
| Observed In: | |
| Stage: | Pec-fin |
Phenotype Detail
Acknowledgments
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Reprinted from Developmental Cell, 32(5), Ristori, E., Lopez-Ramirez, M.A., Narayanan, A., Hill-Teran, G., Moro, A., Calvo, C.F., Thomas, J.L., Nicoli, S., A Dicer-miR-107 Interaction Regulates Biogenesis of Specific miRNAs Crucial for Neurogenesis, 546-60, Copyright (2015) with permission from Elsevier. Full text @ Dev. Cell