FIGURE

Fig. S5

ID

ZDB-FIG-150423-19

Publication

Ristori et al., 2015 - A Dicer-miR-107 Interaction Regulates Biogenesis of Specific miRNAs Crucial for Neurogenesis

Other Figures

All Figure Page

Back to All Figure Page

Fig. S5

miR-9 overexpression strategy and stabilization of dicer, miR-9 and ngn1 hindbrain expression upon dicer-3′UTR protection. Related to Figure 7.

(A) Cartoon describing the strategy adopted for the inducible miR-9 gain-of-function experiment (see Experimental Procedures for details).

(B) Fluorescent and bright-field images of whole-mount zebrafish embryos at 48 hpf injected with the Hsp70-miR-9mCherry plasmid at the one-cell stage and heatshocked at 35 hpf for 15 min at 42°C. Anterior is to the left.

Expression Data

Gene:	neurog1
Fish:	WT WT + MO4-dicer1 + MO5-dicer1
Knockdown Reagents:	MO4-dicer1 MO5-dicer1
Anatomical Term:	hindbrain
Stage:	Pec-fin

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO4-dicer1 + MO5-dicer1
Knockdown Reagents:	MO4-dicer1 MO5-dicer1
Observed In:	hindbrain neurogenesis
Stage:	Pec-fin

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Cell, 32(5), Ristori, E., Lopez-Ramirez, M.A., Narayanan, A., Hill-Teran, G., Moro, A., Calvo, C.F., Thomas, J.L., Nicoli, S., A Dicer-miR-107 Interaction Regulates Biogenesis of Specific miRNAs Crucial for Neurogenesis, 546-60, Copyright (2015) with permission from Elsevier. Full text @ Dev. Cell