ZFIN ID: ZDB-FIG-150422-42
Mackay et al., 2015 - Vitamin K reduces hypermineralisation in zebrafish models of PXE and GACI. Development (Cambridge, England)   142:1095-101 Full text @ Development
Anatomical Terms:
Stage Range: Day 4 to Days 14-20

Fig. 3

abcc6a is expressed at sites of mineralisation, but not the liver. (A) abcc6a transcripts are detected near the opercula (op) of 5dpf embryos. (B) abcc6b expression appears in the opercula (op), parasphenoid (ps), cleithrum (cl) and cartilage of the ear. Neither gene is detected in the liver, in contrast to (C) fetuin-A (ahsg). A,B,C, lateral views; A′,B′,C′, ventral views. (D) A transgenic reporter for abcc6a in a 7dpf embryo stained with Alizarin Red. GFP is seen in the notochord (arrowhead), operculum (D′) and cleithrum (D′′). (E,F) The abcc6a transgenic reporter in an embryo also expressing the osteoblast marker osterix:GFP, demonstrating abcc6a expression in some osteoblasts of the operculum. Expression can also be seen in the neural tube (arrowhead in E). (G) In juvenile (20dpf) vertebrae, abcc6a is expressed in the centra, whereas osx is expressed in the arches. (H) Some abcc6a+ osteoblasts also co-express the mature osteoblast marker osteocalcin:GFP. Dotted outline approximates the extent of the operculum at this stage. (I) In juvenile zebrafish, abcc6a is expressed in the intervertebral disc region, craniofacial bone elements and fins. Scale bars: 10┬Ám in F,H.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
abcc6a WT standard conditions Day 5 Not Detected liver ISH
Day 5 opercular flap ISH
abcc6b.1 WT standard conditions Day 4 cleithrum ISH
Day 4 inner ear cartilage tissue ISH
Day 4 Not Detected liver ISH
Day 4 opercular flap ISH
Day 4 parasphenoid ISH
ahsg1 WT standard conditions Day 5 liver ISH
EGFP hu10967Tg; nkuasgfp1aTg standard conditions Day 5 cleithrum IFL
Day 5 notochord IFL
Day 5 opercular flap IFL
GFP hu10967Tg; nkuasrfp1aTg; zf132Tg standard conditions Day 6 cleithrum IFL
Day 6 opercular flap IFL
Days 14-20 hemal arch osteoblast IFL
Days 14-20 inner ear cartilage tissue IFL
RFP hu10967Tg; nkuasrfp1aTg; zf132Tg standard conditions Day 6 cleithrum IFL
Day 6 opercular flap IFL
Days 14-20 osteoblast IFL
Days 14-20 vertebral column anatomical space IFL
Antibody Labeling Details No data available
Phenotype Details No data available
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