ZFIN ID: ZDB-FIG-150421-9
Navis et al., 2015 - Loss of cftr function leads to pancreatic destruction in larval zebrafish. Developmental Biology   399(2):237-48 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage: Days 14-20
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Days 14-20

Fig. 6

Neutrophil recruitment during pancreatic destruction. (A and B) Neutrophils are absent from representative sections of (A) WT and (B) cftr mutant pancreata at 14 dpf. (C and D) Neutrophils are absent from the (C) WT pancreas at 16 dpf, but present in the (D) cftr mutant pancreas. (E and F) Quantification of (E) the ratio of sections with neutrophils present versus absent to the total number of sections and (F) the average number of neutrophils observed in each pancreas indicating a significant difference in pancreatic neutrophils in the 16 dpf cftr mutant pancreas. The median number of neutrophils in each WT pancreas was 0, ranging from 0 to 1 and cftrpd1049 was 1, ranging from 0 to 5. WT, n=8; cftrpd1049, n=13; *P<0.01. Error bars represent s.e.m. (G and H) DMSO treated (G) WT and (H) cftr mutant samples at 18 dpf expressing ela:GFP in the acinar cells demonstrate typical pancreatic destruction. (I and J) Representative sections of (I) WT and (J) cftr mutant pancreata at 18 dpf treated with PF1052 showing preservation of acinar tissue. (K) Quantification of area of GFP expression divided by total pancreas area for each treatment group showings significantly more area of acinar tissue marked by ela:GFP in PF1052 treated mutants compared with DMSO treated mutants. WT DMSO, n=8; WT PF1052, n=7; cftrpd1049 DMSO, n=8; cftrpd1049, n=12; *P<0.01. Error bars represent s.e.m. Arrows indicate neutrophils. Scale bars=50 ┬Ám.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed cftrpd1049/pd1049; nz50Tg standard conditions Days 14-20 neutrophil IFL
nz50Tg standard conditions Days 14-20 neutrophil IFL
EGFP cftrpd1049/pd1049; gz15Tg control Days 14-20 pancreatic acinar cell IFL
cftrpd1049/pd1049; gz15Tg chemical treatment: pharmaceutical Days 14-20 pancreatic acinar cell IFL
gz15Tg control Days 14-20 pancreatic acinar cell IFL
gz15Tg chemical treatment: pharmaceutical Days 14-20 pancreatic acinar cell IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
cftrpd1049/pd1049; gz15Tg control Days 14-20 pancreas degenerate, abnormal
Days 14-20 pancreatic acinar cell decreased amount, abnormal
cftrpd1049/pd1049; gz15Tg chemical treatment: pharmaceutical Days 14-20 pancreas degenerate, abnormal
Days 14-20 pancreatic acinar cell decreased amount, abnormal
cftrpd1049/pd1049; nz50Tg standard conditions Days 14-20 neutrophil migration increased occurrence, abnormal
Days 14-20 pancreas neutrophil mislocalised, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 399(2), Navis, A., Bagnat, M., Loss of cftr function leads to pancreatic destruction in larval zebrafish, 237-48, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.