FIGURE

Fig. 4

ID

ZDB-FIG-150326-34

Publication

Dyer et al., 2014 - Specification of sensory neurons occurs through diverse developmental programs functioning in the brain and spinal cord

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Fig. 4

Neural crest and RB neuron development is Notch-dependent, but MTN and nTPC neurons do not require Notch signalling for their development. Embryos treated with 100 µM DAPT or DMSO from 30% epiboly were fixed at 24 hpf (A,B,E,F) or 19 hpf (C,D,G,H) and processed by in situ hybridisation with probes for isl1 (A,B,E,F) or crestin (C,D,G,H). In animals treated with DAPT, lateral aspects of the spinal cord reveal that there are many more RB neurons (arrows) relative to DMSO treated controls (A,B). Commensurate with this, there are less migrating neural crest cells (arrows) at spinal cord levels in DAPT-treated animals relative to controls (C,D). Dorsal views of the brain reveal that there are no obvious changes to nTPC (asterisk) or MTN (arrowheads) neurons in DAPT-treated embryos (E,F). However, lateral views of the head reveal that there are less migrating mesencephalic neural crest cells (arrows) in DAPT-treated animals relative to DMSO treated controls (G,H). Quantification of neurons revealed that DAPT treatment caused a significant reduction of RB neurons (**P<0.01), but no significant change (NS) to MTN or nTPC numbers. Scale bars = 100 µm (A–H).

Expression Data

Genes:	crestin isl1a
Fish:	WT
Condition:	chemical treatment: DAPT
Anatomical Terms:	epiphysis mesencephalic nucleus of trigeminal nerve neural crest nucleus of the caudal commissure Rohon-Beard neuron
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT
Condition:	chemical treatment: DAPT
Observed In:	mesencephalic nucleus of trigeminal nerve neural crest cell migration nucleus of the caudal commissure Rohon-Beard neuron
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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