|ZFIN ID: ZDB-FIG-150319-9|
|Stage Range:||Day 5 to Adult|
Fig. 5 Analysis of the eurydendroid-specific GFF line, hspzGFFgDMC156A. (A–G, L–P) The hspzGFFgDMC156A line was crossed with UAS:GFP. Larvae at 5 dpf (A–G) and adult brain sections (L–P) were stained with anti-GFP (green) and anti-Pvalb7 (magenta) antibodies. (A–C) Dorsal views with anterior to the left. (D) Cross-section image of the dotted box in (C). (E) Dorso-anterior view. (F, G) Dorsal (F) and lateral (G) views of eurydendroid cell axons, with anterior to the left. The eurydendroid axons are marked by a dotted line with an arrowhead in F and G. The midlines are indicated by dotted lines in E and F. (L–P) GFP and Pvalb7 expression in the cerebellum of hspzGFFgDMC156A; UAS:GFP adults. (N–P) High magnification views of the box in M. GFP-expressing cells received the axons of Pvalb7+ Purkinje cells (marked by asterisks in N–P). (H–K, Q–S) Co-expression of GFP and RFP in hspzGFFgDMC156A; UAS:RFP; olig2:EGFP eurydendroid cells. The 5-dpf larvae (H–K) and adult brain sections (Q–S) were stained with anti-GFP (green) and anti-RFP (magenta) antibodies. (I–K) High magnification views of the box in H. (Q–S) Sagittal sections. Some of the RFP+ cells were also GFP+ (marked by arrowheads in I–K, Q–S). Projection views (A–H), optical sections (I–K), and histological sections (L–S). Scale bars: 100 µm in A (applied to A–C); 20 µm in D; 50 µm in F; 50 µm in G; 20 µm in H; 20 µm in I (applied to I–K); 200 µm in L (applied to L, M); 50 µm in P (applied to N–P); 50 µm in S (applied to Q–S).
|EGFP||nkhspGFFDMC156AEt; nkuasgfp1aTg||heat shock||Day 5||eurydendroid cell||IHC|
|nkhspGFFDMC156AEt; nkuasrfp1aTg; vu12Tg||heat shock||Day 5||eurydendroid cell||IHC|
|RFP||nkhspGFFDMC156AEt; nkuasrfp1aTg; vu12Tg||heat shock||Day 5||eurydendroid cell||IHC|
|Ab1-pvalb7||IHC||nkhspGFFDMC156AEt; nkuasgfp1aTg||heat shock||Day 5||Purkinje cell|
This image is the copyrighted work of the attributed author or publisher, and
ZFIN has permission only to display this image to its users.
Additional permissions should be obtained from the applicable author or publisher of the image.
Reprinted from Developmental Biology, 397(1), Takeuchi, M., Matsuda, K., Yamaguchi, S., Asakawa, K., Miyasaka, N., Lal, P., Yoshihara, Y., Koga, A., Kawakami, K., Shimizu, T., Hibi, M., Establishment of Gal4 transgenic zebrafish lines for analysis of development of cerebellar neural circuitry, 1-17, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.