ZFIN ID: ZDB-FIG-150319-9
Takeuchi et al., 2015 - Establishment of Gal4 transgenic zebrafish lines for analysis of development of cerebellar neural circuitry. Developmental Biology   397(1):1-17 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Condition:
Anatomical Terms:
Stage Range: Day 5 to Adult

Fig. 5 Analysis of the eurydendroid-specific GFF line, hspzGFFgDMC156A. (A–G, L–P) The hspzGFFgDMC156A line was crossed with UAS:GFP. Larvae at 5 dpf (A–G) and adult brain sections (L–P) were stained with anti-GFP (green) and anti-Pvalb7 (magenta) antibodies. (A–C) Dorsal views with anterior to the left. (D) Cross-section image of the dotted box in (C). (E) Dorso-anterior view. (F, G) Dorsal (F) and lateral (G) views of eurydendroid cell axons, with anterior to the left. The eurydendroid axons are marked by a dotted line with an arrowhead in F and G. The midlines are indicated by dotted lines in E and F. (L–P) GFP and Pvalb7 expression in the cerebellum of hspzGFFgDMC156A; UAS:GFP adults. (N–P) High magnification views of the box in M. GFP-expressing cells received the axons of Pvalb7+ Purkinje cells (marked by asterisks in N–P). (H–K, Q–S) Co-expression of GFP and RFP in hspzGFFgDMC156A; UAS:RFP; olig2:EGFP eurydendroid cells. The 5-dpf larvae (H–K) and adult brain sections (Q–S) were stained with anti-GFP (green) and anti-RFP (magenta) antibodies. (I–K) High magnification views of the box in H. (Q–S) Sagittal sections. Some of the RFP+ cells were also GFP+ (marked by arrowheads in I–K, Q–S). Projection views (A–H), optical sections (I–K), and histological sections (L–S). Scale bars: 100 µm in A (applied to A–C); 20 µm in D; 50 µm in F; 50 µm in G; 20 µm in H; 20 µm in I (applied to I–K); 200 µm in L (applied to L, M); 50 µm in P (applied to N–P); 50 µm in S (applied to Q–S).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP nkhspGFFDMC156AEt; nkuasgfp1aTg heat shock Day 5 eurydendroid cell IHC
Day 5 eurydendroid cell axon IHC
Adult eurydendroid cell IHC
nkhspGFFDMC156AEt; nkuasrfp1aTg; vu12Tg heat shock Day 5 eurydendroid cell IHC
Day 5 eurydendroid cell axon IHC
Adult eurydendroid cell IHC
Adult eurydendroid cell axon IHC
RFP nkhspGFFDMC156AEt; nkuasrfp1aTg; vu12Tg heat shock Day 5 eurydendroid cell IHC
Adult eurydendroid cell IHC
Adult eurydendroid cell axon IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-pvalb7 IHC nkhspGFFDMC156AEt; nkuasgfp1aTg heat shock Day 5 Purkinje cell
IHC Adult Purkinje cell
IHC Adult Purkinje cell axon
Phenotype Details No data available
Acknowledgments:
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Reprinted from Developmental Biology, 397(1), Takeuchi, M., Matsuda, K., Yamaguchi, S., Asakawa, K., Miyasaka, N., Lal, P., Yoshihara, Y., Koga, A., Kawakami, K., Shimizu, T., Hibi, M., Establishment of Gal4 transgenic zebrafish lines for analysis of development of cerebellar neural circuitry, 1-17, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.