FIGURE

Fig. 7

ID

ZDB-FIG-150317-38

Publication

Gutzman et al., 2015 - Non-muscle myosin IIA and IIB differentially regulate cell shape changes during zebrafish brain morphogenesis

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Fig. 7

Non-muscle myosin IIA and IIB protein localization. (A-C) NMIIA and NMIIB antibody staining in 24 ss embryos injected with control MO. (A) NMIIA antibody (green), (B) NMIIB antibody (red), (C) overlay of A and B. (D) Overlay of NMIIA and NMIIB immunostaining in a 24 ss mypt1 MO injected embryo. (E) NMIIA staining with myh10 MO knockdown. (F) NMIIB staining with myh9b MO knockdown. (G-H) No primary control from Alexa 488 and Alexa 555 secondary antibodies. Results are representative of at least three independent experiments. Control MO, n=8; mypt1 MO, n=7; myh10 MO, n=14; myh9b MO, n=19; Alexa 488 no primary control, n=5; Alexa 555 no primary control, n=5.

Expression Data

Antibodies:	Ab2-myh9 Ab2-myh10
Fish:	AB AB + MO1-myh10 AB + MO2-ppp1r12a AB + MO3-myh9a
Knockdown Reagents:	MO1-myh10 MO2-ppp1r12a MO3-myh9a
Anatomical Term:	midbrain hindbrain boundary neuroepithelial cell
Stage:	20-25 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 397(1), Gutzman, J.H., Sahu, S.U., Kwas, C., Non-muscle myosin IIA and IIB differentially regulate cell shape changes during zebrafish brain morphogenesis, 103-15, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.