Fig. 5

myh10 is required for regulation of cell width in the MHB during morphogenesis. (A-E) Live confocal images showing one side of the MHB region of 24 ss zebrafish embryos injected with mGFP and coinjected with control MO (A), myh9a MO (B), myh9b MO (C), myh10 MO (D), or mypt1 MO (E). Single representative cells are outlined in each panel. (F) Quantification of cell width. (G) Quantification of cell area. One-way ANOVA with multiple t-test comparisons was performed to determine significance between control and test groups. Asterisks indicate p<0.001. Double asterisks indicate p<0.05. Results are shown as±s.e.m. 6 cells per embryo (n) were outlined: 2 cells at the MHBC, 2 cells 40 µm posterior to the MHBC, and 2 cells within the 40 µm region between the MHBC and posterior. Cells were chosen based on the ability to see an entire cell from apical to basal in a single z-section. Control MO, n=9; myh9a MO, n=9; myh9b MO, n=14; myh10 MO, n=9; mypt1 MO, n=9. Scale bars: 25 µm.