FIGURE
Fig. 2
- ID
- ZDB-FIG-150316-24
- Publication
- Irion et al., 2014 - Precise and efficient genome editing in zebrafish using the CRISPR/Cas9 system
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Fig. 2
Homology-directed repair at the alb locus. (A) Donor DNAs used. The PCR fragments were also cloned into pGEM-T and injected as circular DNA molecules; the construct with the two CRISPR target sites is depicted. (B-D) Dorsal views of larvae 5dpf: uninjected albb4 control (B), low efficiency repair using linear donor DNA (C) and high efficiency repair using circular donor DNA (D). (E,F) Two examples of adult F0 fish showing pigmented melanophores as a consequence of HDR in melanophore stem cells. |
Expression Data
Expression Detail
Antibody Labeling
Phenotype Data
| Fish: | |
|---|---|
| Observed In: | |
| Stage: | Day 5 |
Phenotype Detail
Acknowledgments
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