ZFIN ID: ZDB-FIG-150316-23
Irion et al., 2014 - Precise and efficient genome editing in zebrafish using the CRISPR/Cas9 system. Development (Cambridge, England)   141(24):4827-30 Full text @ Development
Knockdown Reagent:
Observed In:
Stage: Day 5

Fig. 1

CRISPR-mediated alb knockout. (A) Schematic representation of the alb locus. The gene consists of seven exons (introns are not drawn to scale). The mutation albb4 introduces a premature stop codon into exon 6. (B) The sequence of exon 6 (in capitals). The CRISPR target site, PAM motif and the albb4 mutation are indicated; the SNPs introduced into the donor DNA fragments are shown beneath the CRISPR target site. (C-F) Dorsal views of larvae at 5dpf: uninjected control wild type (C) and albb4 (D) larvae, and injected wild-type larvae with moderate (E) and good (F) alb knockout efficiency.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + CRISPR1-slc45a2 standard conditions Day 5 melanocyte decreased pigmentation, abnormal
slc45a2b4/b4 standard conditions Day 5 melanocyte decreased pigmentation, abnormal
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development