|ZFIN ID: ZDB-FIG-150316-23|
CRISPR-mediated alb knockout. (A) Schematic representation of the alb locus. The gene consists of seven exons (introns are not drawn to scale). The mutation albb4 introduces a premature stop codon into exon 6. (B) The sequence of exon 6 (in capitals). The CRISPR target site, PAM motif and the albb4 mutation are indicated; the SNPs introduced into the donor DNA fragments are shown beneath the CRISPR target site. (C-F) Dorsal views of larvae at 5dpf: uninjected control wild type (C) and albb4 (D) larvae, and injected wild-type larvae with moderate (E) and good (F) alb knockout efficiency.
|WT + CRISPR1-slc45a2||standard conditions||Day 5||melanocyte decreased pigmentation, abnormal|
|slc45a2b4/b4||standard conditions||Day 5||melanocyte decreased pigmentation, abnormal|
|Acknowledgments:||ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development|