|ZFIN ID: ZDB-IMAGE-150406-53|
Homology-directed repair at the alb locus. (A) Donor DNAs used. The PCR fragments were also cloned into pGEM-T and injected as circular DNA molecules; the construct with the two CRISPR target sites is depicted. (B-D) Dorsal views of larvae 5dpf: uninjected albb4 control (B), low efficiency repair using linear donor DNA (C) and high efficiency repair using circular donor DNA (D). (E,F) Two examples of adult F0 fish showing pigmented melanophores as a consequence of HDR in melanophore stem cells.
|Acknowledgments:||ZFIN wishes to thank the journal for permission to reproduce figures from this article. Please note that this material may be protected by copyright.|