ZFIN ID: ZDB-FIG-150313-9
Casari et al., 2014 - A Smad3 transgenic reporter reveals TGF-beta control of zebrafish spinal cord development. Developmental Biology   396(1):81-93 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage: Prim-5

Fig. 7 In vivo blocking of Smad3/TGFbeta; signaling impairs neuronal differentiation during early embryonic development. Confocal Z-stack tail images of 24 hpf transgenic embryos expressing GFP under control of neuronal promoter: ngn1, neuroD, and mnx1. Embryos were injected with morpholinos for smad3a at 1–2 cell stage or treated at 2 hpf with Alk5-inhibitor LY364947. As shown with an arrowhead, morpholinos and drug treatment give similar results: increase of proliferative cells (ngn1) at tail tip, decrease of differentiating cells (neuroD) and reduction of late differentiating motor neurons (mnx1). ngn1 is Tg(ngn1:GFP)sb1. neuroD is Tg(2.4 kb neurod:EGFP). mnx1 is Tg(hlxb9:GFP)ml2. Quantification of each frame is shown in Supplemental Table S3. Scale bar is 100 µm in all the images.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP ia50Tg control Prim-5 caudal fin neuron IFL
ia50Tg chemical treatment: LY 364947 Prim-5 caudal fin neuron IFL
ia50Tg + MO1-smad3a standard conditions Prim-5 caudal fin neuron IFL
GFP ml2Tg control Prim-5 motor neuron IFL
ml2Tg chemical treatment: LY 364947 Prim-5 motor neuron IFL
ml2Tg + MO1-smad3a standard conditions Prim-5 motor neuron IFL
sb1Tg control Prim-5 caudal fin neuron IFL
sb1Tg chemical treatment: LY 364947 Prim-5 caudal fin neuron IFL
sb1Tg + MO1-smad3a standard conditions Prim-5 caudal fin neuron IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ml2Tg chemical treatment: LY 364947 Prim-5 motor neuron decreased amount, abnormal
ml2Tg + MO1-smad3a standard conditions Prim-5 motor neuron decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 396(1), Casari, A., Schiavone, M., Facchinello, N., Vettori, A., Meyer, D., Tiso, N., Moro, E., Argenton, F., A Smad3 transgenic reporter reveals TGF-beta control of zebrafish spinal cord development, 81-93, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.