FIGURE

Fig. S7

ID

ZDB-FIG-150313-14

Publication

Casari et al., 2014 - A Smad3 transgenic reporter reveals TGF-beta control of zebrafish spinal cord development

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Fig. S7

phospho-Smad3 expression pattern correlates with reporter expression. Brightfield images of immunohistochemistry for phospho-Smad3 performed on wild-type embryos at the following developmental stages: 50 % epiboly, tail bud, 13, 15, 24, 36 and 48 hpf. During gastrulation the maternal derived transcription factor is active in the anterior part of the animal pole. It is absent at the end of the gastrulation and it appears again during late somitogenesis (13 hpf) in the tail, eyes and cardiac mesoderm. At 15 hpf it is extended to tail mesoderm. At 24 hpf a weak signal is visible in the cloaca and head. At 48 hpf tail mesoderm signal disappears, while fin buds become positive.

Expression Data

Antibody:	Ab2-smad3
Fish:	WT
Anatomical Terms:	caudal fin cloaca fin bud head heart primordium immature eye spinal cord tail bud tail bud mesoderm whole organism
Stage Range:	50%-epiboly to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 396(1), Casari, A., Schiavone, M., Facchinello, N., Vettori, A., Meyer, D., Tiso, N., Moro, E., Argenton, F., A Smad3 transgenic reporter reveals TGF-beta control of zebrafish spinal cord development, 81-93, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.