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ZFIN ID: ZDB-FIG-150312-46
Feng et al., 2014 - Negative feedback regulation of Wnt signaling via N-linked fucosylation in zebrafish. Developmental Biology   395(2):268-86 Full text @ Dev. Biol.
Anatomical Terms:
Stage: Shield

Fig. 9 The interplay of Wnt8a signaling, slc35c1-mediated GDP-Fuc transport and N-linked fucosylation. (A) qPCR results show the relative expression of slc35c1 and slc35c2 in zebrafish embryos treated with wnt8a or DN-wnt8a mRNA and untreated control sphere stage (4hpf) embryos. All results are normalized to odc1. Expression in WT is defined as 100%. (B-C) Confocal images of slc35c1 fluorescent in situ show positive correlation between slc35c1 expression and β-catenin. Red: β-catenin protein staining. Green: in situ hybridization using slc35C1 antisense probe in (B) or slc35c1 sense probe in (C). Blue: nuclei. (D) A schematic model depicts the relationship between Slc35c1 and Wnt8a. Wnt8a activated canonical Wnt signaling enhances slc35c1 expression. Increased slc35c1 enhances N-linked fucosylation, which inhibits Wnt/β-catenin/TCF signaling during both blastula and gastrula stages. Therefore, these components comprise a negative regulatory loop to control Wnt signaling through N-fucosylation. Note: Bar=300 ┬Ám.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
slc35c1 AB standard conditions Shield whole organism RTPCR
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-ctnnb IHC AB standard conditions Shield anatomical structure
Phenotype Details No data available
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 395(2), Feng, L., Jiang, H., Wu, P., Marlow, F.L., Negative feedback regulation of Wnt signaling via N-linked fucosylation in zebrafish, 268-86, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.