ZFIN ID: ZDB-FIG-150312-41
Feng et al., 2014 - Negative feedback regulation of Wnt signaling via N-linked fucosylation in zebrafish. Developmental Biology   395(2):268-86 Full text @ Dev. Biol.
Anatomical Term:
Stage Range: 30%-epiboly to Day 4
Knockdown Reagent:
Observed In:
Stage: Shield

Fig. 1 slc35c1 enhances the level of N-linked fucosylation expression in zebrafish embryos. (A) Schematic illustration of fucose biosynthetic pathways. Slc35c1 transports GDP-Fuc into the late secretary pathway (mainly the Golgi apparatus). (B) slc35c1 mRNA expression during zebrafish development as quantified by qPCR. The blue bars show slc35c1 relative expression during development and the red dots show the qPCR cycle number of the control gene odc1. (C) Western blot analysis of lysates of 5hpf zebrafish embryos expressing mouse Slc35c1-myc fusion protein. (D) Western blot analysis of global fucosylation levels at shield stage (6hpf) in embryos microinjected with gmds MO or mouse slc35c1-myc mRNA in the presence or absence of GDP-Fuc before and after PNGase F digestion. Proteins were resolved by SDS-PAGE and IB with AAL followed by anti-biotin-HRP in (D) or by streptavidin-Alexa488 for the quantification in (E). N-fucosylation: N-linked fucosylation; O-fucosylation: fucose in mucin and O-fucosylated proteins.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
slc35c1 AB standard conditions 30%-epiboly whole organism RTPCR
Sphere whole organism RTPCR
Bud whole organism RTPCR
5-9 somites whole organism RTPCR
14-19 somites whole organism RTPCR
Prim-15 whole organism RTPCR
Long-pec whole organism RTPCR
Protruding-mouth whole organism RTPCR
Day 4 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-gmds standard conditions Shield fucosylation decreased process quality, abnormal
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Reprinted from Developmental Biology, 395(2), Feng, L., Jiang, H., Wu, P., Marlow, F.L., Negative feedback regulation of Wnt signaling via N-linked fucosylation in zebrafish, 268-86, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.