FIGURE

Fig. 8

ID

ZDB-FIG-150312-18

Publication

Lush et al., 2014 - ErbB expressing Schwann cells control lateral line progenitor cells via non-cell-autonomous regulation of Wnt/beta-catenin

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Fig. 8

Wnt/β-catenin signaling is required for proliferation of interneuromast cells in the absence of ErbB signaling.

Control sibling, Tg(sox10:DNerbb4), Tg(hsp70l:dkk1b) or Tg(sox10:DNerbb4)/Tg(hsp70l:dkk1b), all with Tg(SqET20:gfp) in the background, were heat shocked at 32 hpf then placed in BrdU solution. Fish were fixed at 48 hpf and processed for BrdU and GFP immunohistochemistry (A–D). Compared to control siblings (A), Tg(sox10:DNerbb4) (B) show a strong increase in BrdU incorporation into Tg(SqET20:gfp) positive interneuromast cells. BrdU incorporation in Tg(sox10:DNerbb4) is blocked by over expression of Dkk1b (D). (E) Quantification of BrdU index shows no difference between control and Tg(hsp70l:dkk1b) (Student’s t-test, p=0.6) but a large decrease between Tg(sox10:DNerbb4) and Tg(sox10:DNerbb4)/Tg(hsp70l:dkk1b) (Student’s t-test, p=3.4 E⁸). (F) Quantification of Tg(SqET20:gfp) cell number again shows no difference between control and Tg(hsp70l:dkk1b) (Student’s t-test, p=0.14) but a large decrease between Tg(sox10:DNerbb4) and Tg(sox10:DNerbb4)/Tg(hsp70l:dkk1b) (Student’s t-test, p=1 E⁴).

Expression Data

Gene:	EGFP
Fish:	sqet20Et psi3Tg; sqet20Et psi3Tg; sqet20Et; w32Tg sqet20Et; w32Tg
Condition:	heat shock
Anatomical Term:	interneuromast cell
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	psi3Tg; sqet20Et
Observed In:	interneuromast cell interneuromast cell cell population proliferation
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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