FIGURE

Fig. 8

ID

ZDB-FIG-150304-31

Publication

Campbell et al., 2013 - Local caspase activation interacts with Slit-Robo signaling to restrict axonal arborization

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Fig. 8

Caspase-3 interacts with Slit1a-Robo2 signaling to promote arbor dynamics. (A, C, E, G, and I) Tracings of arbors at 80–85 hpf expressing YFP-Rab3 for slit1a transMO1 (low dose; A), robo2SBMO (low dose; C), casp-3aMO1 (low dose; E), slit1a transMO1 (low dose); casp-3aMO1 (lose dose; G), and robo2SBMO (low dose); casp-3aMO1 (low dose; I) morphant arbors every hour during a 5-h time-lapse imaging. Dorsal views are shown. Black, stable branch tips; red, eliminated tips; green, added tips; blue, tips that were added then eliminated during imaging. (B, D, F, H, and J) The proportion of branch tips in each category. These graphs are derived from analysis of a single arbor in a single experiment. (K, L, O, and P) Branch tip additions (K), retractions (L), presynaptic puncta gain (O), and puncta loss (P) are presented as percentages of initial branch tip or puncta number. (M and N) Total branch tip lifetime (M) and the percentage of branch tips present throughout the imaging period (N). (Q and R) Total puncta lifetime (Q) and the percentage of puncta present throughout the imaging period (R). A minimum of five RGC arbors were analyzed per condition. Dorsal views are shown, and anterior is up. Error bars represent SEMs. *, P < 0.05; **, P < 0.01; ***, P < 0.001. Bar, 10 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	robo2^{ti272z/ti272z} WT + MO1-casp3a + MO1-slit1a WT + MO1-casp3a + MO3-robo2
Knockdown Reagents:	MO1-casp3a MO1-slit1a MO3-robo2
Observed In:	retinal ganglion cell axon collateral retinal ganglion cell collateral sprouting in absence of injury retinal ganglion cell neuron remodeling
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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