FIGURE

Fig. 4

ID

ZDB-FIG-150304-27

Publication

Campbell et al., 2013 - Local caspase activation interacts with Slit-Robo signaling to restrict axonal arborization

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

Capase-3, Caspase-9, and p38 MAPK promote RGC axon arbor dynamics. (A, C, E, G, and I) Tracings of arbors at 80–85 hpf expressing YFP-Rab3. Images represent WT (A), coMO (C), casp-3aMO1 (E), casp-9MO1 (G), and p38mapkMO1 (I) morphant arbors every hour during a 5-h time-lapse imaging. All show dorsal views. Black, stable branch tips; red, eliminated tips; green, added tips; blue, tips that were added then eliminated during imaging. (B, D, F, H, and J). The proportion of branch tips in each category. These graphs are derived from analysis of a single arbor in a single experiment. (K and L) Branch tip additions (K) and retractions (L) are shown in the graphs. (N and O) Presynaptic puncta gain (N) and loss (O) are presented as a percentage of initial branch tip or presynaptic puncta, respectively. (M and P) Total branch tip (M) and presynaptic puncta lifetimes (P) include all branch tips or presynaptic puncta present at one or more time points. (Q and R) Total branch tip (Q) and presynaptic puncta lifetime distribution (R) are shown. 9–13 RGC arbors were analyzed per condition. Tracings are dorsal views; anterior is up. Error bars represent SEMs. *, P < 0.05; **, P < 0.01; ***, P < 0.001. Bar, 10 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-casp3a WT + MO1-casp9 WT + MO1-mapk14a
Knockdown Reagents:	MO1-casp3a MO1-casp9 MO1-mapk14a
Observed In:	retinal ganglion cell axon collateral retinal ganglion cell collateral sprouting in absence of injury retinal ganglion cell neuron remodeling
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Biol.