FIGURE

Fig. 7

ID

ZDB-FIG-150304-30

Publication

Campbell et al., 2013 - Local caspase activation interacts with Slit-Robo signaling to restrict axonal arborization

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Fig. 7

p38 MAPK interacts with Slit1a-Robo2 signaling to restrict arbor and presynaptic growth. (A–F) Maximum intensity projections of 80-hpf RGC arbors in the optic tectum expressing YFP-Rab3. coMO (A), p38mapkMO1 (B), slit1a transMO1 (low dose; C), robo2SBMO1 (low dose; D), p38mapkMO1; slit1a transMO1 (low dose; E), and p38mapkMO1; robo2SBMO1 (low dose; F). (G–L) Quantification of arbor parameters: branch tip number (G), arbor area (H), total arbor length (I), arbor order (J), presynaptic puncta number (K), and puncta density (L). 6–20 arbors were analyzed per condition. Dorsal views are shown, and anterior is up. White asterisks indicate the parent axon. Error bars represent SEMs. *, P < 0.05; **, P < 0.01; ***, P < 0.001. Bar, 10 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-mapk14a WT + MO1-slit1a WT + MO3-robo2 WT + MO1-mapk14a + MO1-slit1a WT + MO1-mapk14a + MO3-robo2
Knockdown Reagents:	MO1-mapk14a MO1-slit1a MO3-robo2
Observed In:	retinal ganglion cell axon retinal ganglion cell axon collateral retinal ganglion cell collateral sprouting in absence of injury retinal ganglion cell presynaptic active zone
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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