ZFIN ID: ZDB-FIG-150211-11
So et al., 2014 - FIH-1, a Novel Interactor of Mindbomb, Functions as an Essential Anti-Angiogenic Factor during Zebrafish Vascular Development. PLoS One   9:e109517 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage: Protruding-mouth

Fig. 4

Fih-1 negatively regulates Vegf-aa165 in zebrafish embryos.

(A) At 28 hpf, both fih-1 and vegf-aa165 transcript are selectively expressed within the anterior somites and ventral mesoderm. (B) Attenuation of Fih-1 activity in zebrafish elevates the level of vegf-aa165 expression at 50 hpf. Arrowheads point to vegf-aa165 expression in somites caused by lack of Fih-1 activity. Similarly, the level of vegfaa transcript in 48 hpf control or fih-1 MO-injected embryos was evaluated by quantitative RT-PCR. Error bars are standard deviation (right). (C) Ectopic expression of Fih-1 decreases the level of vegf-aa165 expression in a dose-dependent manner at 28 hpf. (D) Functional relationship between Fih-1 and Vegf-aa165. Ectopic expression of Vegf-aa165 under the regulation of hsp70l promoter caused a similar vascular phenotype as observed in fih-1 MO-injected embryos. Moreover, inhibition of Vegf-A signaling can drastically reduce the exuberant angiogenic sprouts in fih-1 MO-injected embryos at 80 hpf. Arrows point to ectopic sprouts.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
hif1an WT control Prim-5 somite anterior region ISH
Prim-5 ventral mesoderm ISH
vegfaa WT control Prim-5 somite anterior region ISH
Prim-5 ventral mesoderm ISH
Long-pec somite ISH
Long-pec whole organism RTPCR
WT + MO1-hif1an standard conditions Long-pec somite ISH
Long-pec whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ck4Tg; s843Tg standard conditions Protruding-mouth angiogenic sprout mislocalised, abnormal
Protruding-mouth intersegmental vessel angiogenic sprout increased amount, abnormal
Protruding-mouth sprouting angiogenesis process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One