ZFIN ID: ZDB-FIG-150211-11
So et al., 2014 - FIH-1, a Novel Interactor of Mindbomb, Functions as an Essential Anti-Angiogenic Factor during Zebrafish Vascular Development. PLoS One   9:e109517 Full text @ PLoS One
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec
Observed In:
Stage: Protruding-mouth

Fig. 4

Fih-1 negatively regulates Vegf-aa165 in zebrafish embryos.

(A) At 28 hpf, both fih-1 and vegf-aa165 transcript are selectively expressed within the anterior somites and ventral mesoderm. (B) Attenuation of Fih-1 activity in zebrafish elevates the level of vegf-aa165 expression at 50 hpf. Arrowheads point to vegf-aa165 expression in somites caused by lack of Fih-1 activity. Similarly, the level of vegfaa transcript in 48 hpf control or fih-1 MO-injected embryos was evaluated by quantitative RT-PCR. Error bars are standard deviation (right). (C) Ectopic expression of Fih-1 decreases the level of vegf-aa165 expression in a dose-dependent manner at 28 hpf. (D) Functional relationship between Fih-1 and Vegf-aa165. Ectopic expression of Vegf-aa165 under the regulation of hsp70l promoter caused a similar vascular phenotype as observed in fih-1 MO-injected embryos. Moreover, inhibition of Vegf-A signaling can drastically reduce the exuberant angiogenic sprouts in fih-1 MO-injected embryos at 80 hpf. Arrows point to ectopic sprouts.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
hif1an WT control Prim-5 somite anterior region ISH
Prim-5 ventral mesoderm ISH
vegfaa WT control Prim-5 somite anterior region ISH
Prim-5 ventral mesoderm ISH
Long-pec somite ISH
Long-pec whole organism RTPCR
WT + MO1-hif1an standard conditions Long-pec somite ISH
Long-pec whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ck4Tg; s843Tg standard conditions Protruding-mouth angiogenic sprout mislocalised, abnormal
Protruding-mouth intersegmental vessel angiogenic sprout increased amount, abnormal
Protruding-mouth sprouting angiogenesis process quality, abnormal
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One