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ZFIN ID: ZDB-FIG-150108-5
Huang et al., 2013 - Sequential effects of spadetail, one-eyed pinhead and no tail on midline convergence of nephric primordia during zebrafish embryogenesis. Developmental Biology   384(2):290-300 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 5-9 somites to Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage Range: 5-9 somites to Long-pec

Fig. 6

PGP midline convergence phenotypes in spt-/-embryos. ((A)-(C)) Whole-mount in situ hybrydization of wt1a and myoD was stained blue and orange, respectively in 12 (A), 24 (B) and 48 hpf (C) spt-/- embryos. The scale bar indicates 100 ┬Ám; ((A)-(C)) are at the same magnification. All images are dorsal view anterior to the top. (D) Quantification of convergence morphogenetic movements in spt-/- embryos and comparison with WT. The inter distance of PGP was measured at 11 (n=28), 12 (n=32), 15 (n=31), 24 (n=9), 48 (n=15) hpf. The P values of t-test for all 5 stages are smaller than 0.005. The curve fitting (pink) and R-squared value are displayed on the right-hand corner. A turning point (arrow) to divergence movement at 33.5 hpf is generated from an approximation of the stage that has the minimum inter distance of PGP. Error bars represent SD.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
myod1 tbx16b104/b104 standard conditions 5-9 somites adaxial cell ISH
5-9 somites somite ISH
Prim-5 adaxial cell ISH
Prim-5 somite ISH
Long-pec somite ISH
wt1a tbx16b104/b104 standard conditions 5-9 somites pronephric mesoderm ISH
Prim-5 pronephric glomerulus ISH
Long-pec pronephric glomerulus ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
tbx16b104/b104 standard conditions 5-9 somites convergent extension involved in nephron morphogenesis process quality, abnormal
tbx16b104/b104 standard conditions 5-9 somites pronephric glomerulus morphogenesis process quality, abnormal
tbx16b104/b104 standard conditions Prim-5 convergent extension involved in nephron morphogenesis process quality, abnormal
tbx16b104/b104 standard conditions Prim-5 pronephric glomerulus morphogenesis process quality, abnormal
tbx16b104/b104 standard conditions Long-pec convergent extension involved in nephron morphogenesis process quality, abnormal
tbx16b104/b104 standard conditions Long-pec pronephric glomerulus morphogenesis process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 384(2), Huang, C.J., Wilson, V., Pennings, S., MacRae, C.A., and Mullins, J., Sequential effects of spadetail, one-eyed pinhead and no tail on midline convergence of nephric primordia during zebrafish embryogenesis, 290-300, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.