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ZFIN ID: ZDB-FIG-141217-22
Kuo et al., 2014 - Hedgehog-PKA Signaling and gnrh3 Regulate the Development of Zebrafish gnrh3 Neurons. PLoS One   9:e95545 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Knockdown Reagents:
Anatomical Term:
Stage: Long-pec

Fig. 5 Hh-PKA pathway is required for the development of gnrh3 neurons.

All embryos are at 2A–D, gnrh3 neurons were detected by in situ hybridization with the gnrh3 probe in the wildtype (wt), but not in oep, cyc and smu mutants. E–F. Ectopic GFP expression near the olfactory region in embryos injected with (F) shhb, but not (E) shha mRNA. G–H, gnrh3 signals were normal in control (G) tomatide-treated embryos, but were lost in embryos treated with (H) Hh inhibitor cyclopamine (CYA, 100 μM) at 6–10 hpf. I–K, More ectopic gnrh3-expressing cells were detected after co-injection of gsk3b-MO or cnsk1a-MO with PKI mRNA. L, Gsk3b and csnk1a double MO treatment did not increase the number of ectopic gnrh3-expressing cells. The anterior is to the left in all panels.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
gnrh3 WT control Long-pec olfactory bulb ISH
WT standard conditions Long-pec olfactory bulb ISH
WT chemical treatment: Cyclopamine Long-pec Not Detected olfactory bulb ISH
WT + MO1-csnk1a1 + MO2-gsk3b standard conditions Long-pec olfactory bulb ISH
ndr2Df(Chr12:hhex)b16/b16 control Long-pec Not Detected olfactory bulb ISH
smob577/b577 control Long-pec Not Detected olfactory bulb ISH
tdgf1m134/m134 control Long-pec Not Detected olfactory bulb ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One