PUBLICATION

Hedgehog-PKA Signaling and gnrh3 Regulate the Development of Zebrafish gnrh3 Neurons

Authors
Kuo, M.W., Lou, S.W., Chung, B.C.
ID
ZDB-PUB-140601-2
Date
2014
Source
PLoS One   9: e95545 (Journal)
Registered Authors
Chung, Bon-chu
Keywords
none
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Cyclic AMP-Dependent Protein Kinases/metabolism*
  • Fibroblast Growth Factors/metabolism
  • Gene Expression Regulation, Developmental
  • Gonadotropin-Releasing Hormone/genetics
  • Gonadotropin-Releasing Hormone/metabolism*
  • Hedgehog Proteins/metabolism*
  • Neurons/cytology
  • Neurons/enzymology
  • Neurons/metabolism*
  • Pyrrolidonecarboxylic Acid/analogs & derivatives*
  • Pyrrolidonecarboxylic Acid/metabolism
  • Signal Transduction*
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed
24879419 Full text @ PLoS One
Abstract
GnRH neurons secrete GnRH that controls the development of the reproduction system. Despite many studies, the signals controlling the development of GnRH neurons from its progenitors have not been fully established. To understand the development of GnRH neurons, we examined the development of gnrh3-expressing cells using a transgenic zebrafish line that expresses green fluorescent protein (GFP) and LacZ driven by the gnrh3 promoter. GFP and LacZ expression recapitulated that of gnrh3 in the olfactory region, olfactory bulb and telencephalon. Depletion of gnrh3 by morpholinos led to a reduction of GFP- and gnrh3-expressing cells, while over-expression of gnrh3 mRNA increased the number of these cells. This result indicates a positive feed-forward regulation of gnrh3 cells by gnrh3. The gnrh3 cells were absent in embryos that lack Hedgehog signaling, but their numbers were increased in embryos overexpressing shhb. We manipulated the amounts of kinase that antagonizes the Hedgehog signaling pathway, protein kinase A (PKA), by treating embryos with PKA activator forskolin or by injecting mRNAs encoding its constitutively active catalytic subunit (PKA*) and dominant negative regulatory subunit (PKI) into zebrafish embryos. PKA* misexpression or forskolin treatment decreased GFP cell numbers, while PKI misexpression led to ectopic production of GFP cells. Our data indicate that the Hedgehog-PKA pathway participates in the development of gnrh3-expressing neurons during embryogenesis.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes