FIGURE

Fig. S17

ID

ZDB-FIG-140922-30

Publication

Sasaki et al., 2014 - Aberrant Autolysosomal Regulation Is Linked to The Induction of Embryonic Senescence: Differential Roles of Beclin 1 and p53 in Vertebrate Spns1 Deficiency

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Fig. S17

Suppression of spns1-mutant phenotypes by knockdown of the atp6v0c gene in zebrafish embryos. (A) Gross morphology, EGFP-LC3 and LysoTracker intensities in wild-type (wt) and spns1-mutant animals injected with atp6v0c MO (4 ng/embryo) at 48 hpf. Suppression of yolk opacity and SA-β-gal (SABG) by injection of atp6v0c MO in zebrafish embryos was observed at 48 and 60 hpf, respectively. Scale bar, 250 μm. (B) Quantification of the SA-β-gal intensities shown in (A). Quantification of data presented in panel A (n = 10) is shown in the right graph; the number (n) of animals is for each genotype with MO. (C) Effect of the PPIs (omeprazole; OPZ, lansoprazole; LPZ, and pantoprazole; PPZ) on embryonic senescence (SABG; SA-β-gal) in the spns1 mutant at 48 hpf. The drug treatments were done for 12 h from 36 hpf through 48 hpf. Scale bar, 250 μm. Error bars represent the mean ± S.D., *p<0.005; ns, not significant.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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