FIGURE

Fig. S8

ID
ZDB-FIG-140922-24
Publication
Sasaki et al., 2014 - Aberrant Autolysosomal Regulation Is Linked to The Induction of Embryonic Senescence: Differential Roles of Beclin 1 and p53 in Vertebrate Spns1 Deficiency
Other Figures
All Figure Page
Back to All Figure Page
Fig. S8

Impact of Beclin 1 depletion on Spns1 deficiency in the presence or absence of p53. (A) Yolk opaque phenotype of control MO-injected or beclin 1 MO-injected wild-type (spns1+/+;tp53+/+), tp53zdf1/zdf1 (tp53m/m), spns1hi891/hi891 (spns1-/-), and spns1hi891/hi891;tp53zdf1/zdf1 (spns1-/-;tp53m/m) animals is compared at 48 hpf. Opacity is greater in the p53 mutant background with Spns1 deficiency. The attenuated suppressive effect of beclin 1 MO (12 ng/embryo) yolk opacity in spns1hi891/hi891;tp53zdf1/zdf1 animals is shown. Scale bar, 250 μm. (B) spns1hi891/hi891 animals coinjected with beclin 1 MO and p53 MO or beclin 1 MO and inverse-sequence p53 MO (inv. p53 MO; negative control) were assayed for the SA-β-gal detection at 84 hpf. The beclin 1 MO-mediated suppression of SA-β-gal in spns1hi891/hi891 animals was attenuated by p53 MO injection. Scale bar, 250 μm. (C) Quantification of the SAβ-gal intensities shown in (B). Quantification of data presented in panel B (n = 10) is shown in the right graph; the number (n) of animals is for each morphant. Error bars represent the mean ± S.D., *p<0.005; ns, not significant.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.