FIGURE

Fig. 3

ID

ZDB-FIG-140909-12

Publication

Plavicki et al., 2014 - Multiple modes of proepicardial cell migration require heartbeat

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Fig. 3

Heterogeneous tcf21 expression within the developing epicardium. Confocal images of the developing zebrafish epicardium. (A-A”) Lateral view of a 1-week pard3:EGFP; tcf21:DsRed2 heart (n = 10). (A) Epicardial cells are marked with pard3:EGFP (green). (A′) Immunostaining for DsRed2 (red). (A”) Merge of A and A′ with DAPI staining (nuclei; blue). Arrows indicate pard3+/tcf21- epicardial cells. (B-B′) Ventral view of a 2-week cmlc2:EGFP; tcf21:DsRed2 heart (n = 5). (B) Epicardial cells are marked with immunostaining for DsRed2 (red). (B′) cmlc2:EGFP; tcf21:DsRed2 heart with DAPI staining (nuclei; blue). tcf21-/DAPI + epicardial cells (arrows) are seen overlying the myocardium. (C-C”) Ventricular epicardium from a 6-week old zebrafish heart (n = 5). (C) Epicardial cells are marked with pard3:EGFP (green). (C′) Immunostaining for DsRed2 (red). (C”) Merge of C and C′. For all panels: V is ventricle, BA is bulbus arteriosus. Scale bars = 50 microns.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.