|ZFIN ID: ZDB-FIG-140903-33|
S6 kinase phosphorylation and autophagy is induced by reactive oxygen species (ROS).
(A) Confocal microscopy analysis of GFP-LC3 HEK cells transfected with siScr or siRPS19 and either untreated or treated with 10 mM Trolox overnight. Size bars = 10 μM. (B) Quantification of the average number of GFP-LC3 puncta per cell in (A). (C) Western blot analysis of phosphorylated S6 kinase expression in GFP-LC3 HEK transfected with siScr or siRPS19 and either untreated or treated with 10 mM Trolox overnight. (D) Western blot analysis of phosphorylated S6 kinase and phosphorylated AKT substrate expression in 2 dpf zebrafish embryos untreated or treated with 10 mM Trolox overnight. (E) Survival rates of embryos treated overnight with 10 mM Trolox. The results are the compilation of three independent experiments with N = 25 embryos representing each mutation. The statistics shown are compared to wild type. (F) Analysis of p53 stabilization in 2 dpf wild type, rpS7-deficient embryos, p53M214K/M214K mutant embryos, or double mutants (rpS7;p53) by western blotting. (G) Analysis of p53 stabilization by western blotting of zebrafish mutants treated overnight with 100 μM Trolox.
|WT||chemical treatment: Trolox||Long-pec||whole organism||WB|
|rps7hi1034bTg/hi1034bTg||chemical treatment: Trolox||Long-pec||whole organism||WB|
|tp53zdf1/zdf1; rps7hi1034bTg/hi1034bTg||control||Long-pec||whole organism||WB|
|tp53zdf1/zdf1; rps7hi1034bTg/hi1034bTg||chemical treatment: Trolox||Long-pec||whole organism||WB|
|WB||rps3ahi1290Tg/hi1290Tg||chemical treatment: Trolox||Long-pec||whole organism|
|WB||rps7hi1034bTg/hi1034bTg||chemical treatment: Trolox||Long-pec||whole organism|
|WT||chemical treatment: Trolox||Long-pec||whole organism dead, abnormal|
|rpl11hi3820bTg/hi3820bTg||chemical treatment: Trolox||Long-pec||whole organism dead, abnormal|
|rps3ahi1290Tg/hi1290Tg||chemical treatment: Trolox||Long-pec||whole organism viable, abnormal|
|rps7hi1034bTg/hi1034bTg||chemical treatment: Trolox||Long-pec||whole organism semi-viable, abnormal|
|Acknowledgments:||ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.|