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ZFIN ID: ZDB-FIG-140903-33
Heijnen et al., 2014 - Ribosomal Protein Mutations Induce Autophagy through S6 Kinase Inhibition of the Insulin Pathway. PLoS Genetics   10:e1004371 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Condition:
Anatomical Term:
Stage: Long-pec
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Long-pec

Fig. 7

S6 kinase phosphorylation and autophagy is induced by reactive oxygen species (ROS).

(A) Confocal microscopy analysis of GFP-LC3 HEK cells transfected with siScr or siRPS19 and either untreated or treated with 10 mM Trolox overnight. Size bars = 10 μM. (B) Quantification of the average number of GFP-LC3 puncta per cell in (A). (C) Western blot analysis of phosphorylated S6 kinase expression in GFP-LC3 HEK transfected with siScr or siRPS19 and either untreated or treated with 10 mM Trolox overnight. (D) Western blot analysis of phosphorylated S6 kinase and phosphorylated AKT substrate expression in 2 dpf zebrafish embryos untreated or treated with 10 mM Trolox overnight. (E) Survival rates of embryos treated overnight with 10 mM Trolox. The results are the compilation of three independent experiments with N = 25 embryos representing each mutation. The statistics shown are compared to wild type. (F) Analysis of p53 stabilization in 2 dpf wild type, rpS7-deficient embryos, p53M214K/M214K mutant embryos, or double mutants (rpS7;p53) by western blotting. (G) Analysis of p53 stabilization by western blotting of zebrafish mutants treated overnight with 100 μM Trolox.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
tp53 WT control Long-pec whole organism WB
WT chemical treatment: Trolox Long-pec whole organism WB
rps7hi1034bTg/hi1034bTg control Long-pec whole organism WB
rps7hi1034bTg/hi1034bTg chemical treatment: Trolox Long-pec whole organism WB
tp53zdf1/zdf1 control Long-pec whole organism WB
tp53zdf1/zdf1; rps7hi1034bTg/hi1034bTg control Long-pec whole organism WB
tp53zdf1/zdf1; rps7hi1034bTg/hi1034bTg chemical treatment: Trolox Long-pec whole organism WB
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab6-rps6k WB WT control Long-pec whole organism
WB rps3ahi1290Tg/hi1290Tg control Long-pec whole organism
WB rps3ahi1290Tg/hi1290Tg chemical treatment: Trolox Long-pec whole organism
WB rps7hi1034bTg/hi1034bTg control Long-pec whole organism
WB rps7hi1034bTg/hi1034bTg chemical treatment: Trolox Long-pec whole organism
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment: Trolox Long-pec whole organism dead, abnormal
rpl11hi3820bTg/hi3820bTg chemical treatment: Trolox Long-pec whole organism dead, abnormal
rps3ahi1290Tg/hi1290Tg chemical treatment: Trolox Long-pec whole organism viable, abnormal
rps7hi1034bTg/hi1034bTg chemical treatment: Trolox Long-pec whole organism semi-viable, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.