FIGURE

Fig. 2

ID

ZDB-FIG-140811-19

Publication

Ju et al., 2014 - Activation of Sonic hedgehog signaling in neural progenitor cells promotes glioma development in the zebrafish optic pathway

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Fig. 2

Phenotypic characterization of a stable transgenic line. (a) Graphic representation of the DNA constructs used for transgenesis and breeding. (b) A 24h.p.f. embryo expresses Smoa1-EGFP in the forebrain and retinal neuroblasts. A 96h.p.f. double transgenic embryo derived from crossing of the Tg(krt5:Gal4VP16;14 × UAS:smoa1-EGFP) and the Tg(UAS-E1b:nfsB-mCherry)^c264 stable lines expressed GFP (c) and mCherry (d) in the ONs (arrows). (e) A 6-month-old wild-type and (f) a transgenic fish with a gross tumor in its left eye (arrow). (g) Wild-type fish formed a normal optic chiasm, whereas (h) transgenic fish failed to form optic chiasm and showed dramatic expansion of the ONH region (arrow). (i) Dissected brain and eyes showing an enlarged ON associating with a gross eye tumor (arrow).

Expression Data

Genes:	EGFP mCherry
Fish:	sj4Tg c264Tg; sj4Tg
Anatomical Terms:	cranial nerve II forebrain optic cup neuroblast
Stage Range:	Prim-5 to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sj4Tg
Observed In:	cranial nerve II eye optic chiasm optic nerve head
Stage:	Adult

Phenotype Detail

Acknowledgments

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