ZFIN ID: ZDB-FIG-140807-35
Araya et al., 2014 - Mesoderm is required for coordinated cell movements within zebrafish neural plate in vivo. Neural Development   9(1):9 Full text @ Neural Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Condition:
Anatomical Terms:
Stage Range: 20-25 somites to Prim-5
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: 20-25 somites to Prim-5

Fig. 4 Loss of mesoderm, but not Nodal signaling, leads to neural tube defects. (A,B,C,D,E,F,G) Projection of confocal z-series showing dorsal view of 24 hpf zebrafish embryos staining for the apical marker ZO-1 (green). (A) Disrupted ventricular organization of embryo treated with the Nodal inhibitor SB-431542 from the one-cell stage. (B) Treatment with SB-431542 from 70% epiboly does not cause neural tube defects. (C,D) Ventricle organization in Nodal mutants cyc and oep is largely normal. (E,F) Ventricle organization in ntl and syu mutants is normal. (G) Ventricle organization in the endoderm mutant cas mutants is normal. (H) Expression of the Nodal-dependent markers lefty and pitx2 show the SB-431542 drug is an efficient blocker of Nodal signaling. All embryos between 21 and 23 hpf, dorsal view. Arrowhead in (H) indicates asymmetric lefty expression at 21 hpf. (I,I′) Two frames from time-lapse sequence show transplanted MZoep cells (green) integrate and divide to make mirror-image daughters just like host cells in a wild-type embryo at 15 hpf. (J,J′) Two frames at 15 hpf from time-lapse sequence show wild-type cells (green) divide to make mirror-image daughters after transplantation to a MZoep embryo. Unlike divisions in wild-type embryos, however, these divisions can be far from their normal location at the midline. hpf, hours post fertilization; MZoep, maternal-zygotic one-eyed pinhead; wt, wild-type; ZO-1, zonula occludens 1.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
lft1 WT standard conditions 20-25 somites zebrafish anatomical entity ISH
WT chemical treatment: pharmaceutical 20-25 somites Not Detected zebrafish anatomical entity ISH
pitx2 WT standard conditions 26+ somites zebrafish anatomical entity ISH
WT chemical treatment: pharmaceutical 26+ somites Not Detected zebrafish anatomical entity ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-tjp1 IHC WT chemical treatment: pharmaceutical Prim-5 neural tube
IHC Prim-5 ventricular system apical surface
IHC ndr2m294/m294 standard conditions Prim-5 ventricular system apical surface
IHC shhaDf(Chr07)t4 standard conditions Prim-5 ventricular system apical surface
IHC sox32unspecified/unspecified standard conditions Prim-5 ventricular system apical surface
IHC tbxtab160/b160 standard conditions Prim-5 ventricular system apical surface
IHC tdgf1tz257/tz257 standard conditions Prim-5 ventricular system apical surface
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment: pharmaceutical 20-25 somites nodal signaling pathway decreased process quality, abnormal
26+ somites nodal signaling pathway decreased process quality, abnormal
Prim-5 neural tube morphology, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Neural Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Neural Dev.