ZFIN ID: ZDB-FIG-140807-33
Araya et al., 2014 - Mesoderm is required for coordinated cell movements within zebrafish neural plate in vivo. Neural Development   9(1):9 Full text @ Neural Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Condition:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: 14-19 somites to Prim-5

Fig. 2 Ectopic divisions do not generate the abnormal neural tube in MZoep embryos. (A) Location and orientation of divisions monitored over a 2-hour time-lapse period of neural rod development in wild-type embryo by analyzing the expression of apical marker Pard3-GFP (green) and H2B-RFP mRNA to label nuclei (not included in image for clarity, red dumbbells indicate location and orientation of dividing cells). Wild-type neural progenitor divisions are strongly orientated along the mediolateral axis of the developing neural tube. Yellow dots outline the rod. (B) Location and orientation of divisions monitored over a 2-hour time-lapse period of neural rod development in MZoep embryo. (C,D) Orientation plots of divisions in wild-type and MZoep embryos. (E,F,G) Dorsal projections of ventricle morphology (ZO-1, green) at 24 hpf in wild-type, MZoep embryos and MZoep embryos treated with CDIs (hydroxyurea and aphidicolin). Blocking division does not rescue ventricle morphology. PH3 staining of mitotic figures (purple) used to calculate efficiency of division block. (H) Transverse section of brain from MZoep embryo treated with division blockers. (I) Quantification of divisions in wild-type, MZoep and MZoep division blocked embryos. Number of cell divisions between wild-type 194 and MZoep 206, P = 0.5129 Student’s t-test and between MZoep 206 and MZoep + CDI 39, ***P <0.0001 Student’s t-test. In (E), (F) and (G), equivalent tissue volumes were used (360 µm in length x 130 µm in width x 90 µm in depth). In (I), error bars indicate SEM. CDI, cell division inhibitor; H2B-RFP, histone H2B/red fluorescent protein fusion; hpf, hours post fertilization; MZoep, maternal-zygotic one-eyed pinhead; Pard3-GFP, green fluorescent protein/polarity protein partitioning defective 3 fusion; PH3, phospho-histone 3 marker; SEM, standard error of the mean; wt, wild-type; ZO-1, zonula occludens 1.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP tdgf1tz257/tz257; knu3Tg chemical treatment: pharmaceutical Prim-5 neural tube IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-tjp1 IHC WT standard conditions Prim-5 ventricular system apical surface
IHC tdgf1tz257/tz257 standard conditions Prim-5 neural tube
IHC tdgf1tz257/tz257 chemical treatment: pharmaceutical Prim-5 neural tube
Phenotype Details
Fish Conditions Stage Phenotype
tdgf1tz257/tz257; knu3Tg standard conditions 14-19 somites - 20-25 somites neural tube cell division cellular spatiotemporal quality, abnormal
14-19 somites - 20-25 somites neural tube morphology, abnormal
tdgf1tz257/tz257; knu3Tg chemical treatment: pharmaceutical Prim-5 neural tube cell division arrested, abnormal
Prim-5 neural tube morphology, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Neural Dev.