Fig. 1
- ID
- ZDB-FIG-140715-28
- Publication
- Bonetti et al., 2014 - Distinct and Overlapping Functions of ptpn11 Genes in Zebrafish Development
- Other Figures
- All Figure Page
- Back to All Figure Page
Expression of zebrafish ptpn11a and ptpn11b mRNA during embryogenesis. (a) RNA was isolated from zebrafish embryos at 4 hpf, 10 hpf, 1 dpf, 2 dpf, 3 dpf and 5 dpf. Reverse transcription PCR was performed to detect the indicated genes; reverse transcriptase was omitted from the reaction as a control (-RT). (b) Quantitative PCR for ptpn11a and ptpn11b gene expression was performed from a pool of wild-type embryos (n = 30) collected at 10 hpf, 1 dpf, 2 dpf, 3 dpf and 5 dpf. The values were normalized using Actin as a control and relative expression is depicted here (value at 5 dpf set to 1.0). Error bars indicate standard error of the mean. (c) Whole-mount in situ hybridization using ptpn11a and ptpn11b specific probes of 4 hpf, 10 hpf, 30 hpf, 2 dpf, 3 dpf and 5 dpf embryos. Lateral and dorsal views are given as indicated. |
Genes: | |
---|---|
Fish: | |
Anatomical Terms: | |
Stage Range: | Sphere to Day 5 |