FIGURE

Fig. S2

ID

ZDB-FIG-140624-9

Publication

O'Brien et al., 2014 - MicroRNA-30a regulates zebrafish myogenesis through targeting the transcription factor Six1

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Fig. S2

Morpholino studies reveal that six1a and six1b function together during zebrafish myogenesis. Live images (A) at 24 hours post fertilization (hpf), anterior to the left, show that lower doses of six1a MO and six1b MO injected alone do not disrupt normal somite morphology individually, only when injected together are U-shaped somites observed (control: n=75/77, 6.5 ng six1a MO: n=61/64, 2.5 ng six1b MO: n=50/56, six1a/b MO: n=100/154). Lateral views of in situ hybridization (B) at 9 somites shows an early delay of tropomyosin-α (tpma) (control: n=25/27, six1a MO: n=21/25, six1b MO: n=20/25, six1a/b MO: n=10/14), which is strongest in the double six1a/b knockdown. Scalebar = 100 µm.

Expression Data

Gene:	tpma
Fish:	WT WT + MO2-six1a WT + MO4-six1b WT + MO2-six1a + MO4-six1b
Knockdown Reagents:	MO2-six1a MO4-six1b
Anatomical Term:	somite
Stage:	5-9 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-six1a + MO4-six1b
Knockdown Reagents:	MO2-six1a MO4-six1b
Observed In:	somite
Stage:	Prim-5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.