FIGURE

Fig. 2

ID

ZDB-FIG-140618-19

Publication

Quillien et al., 2014 - Distinct Notch signaling outputs pattern the developing arterial system

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Fig. 2

Notch activity increases and persists in arterial endothelial cells at later developmental stages. (A-D) Confocal micrographs of Tg(tp1:egfp)^um14 embryos following fluorescence in situ hybridization using riboprobes against egfp (green) and fli1a (red). Lateral views, dorsal is up, anterior to the left. (A,C,D) White arrows denote dorsal aorta. (A) 16 ss embryo; the white box indicates the magnified area shown in B. (B) Yellow arrowheads and white arrows denote endothelial cells with high and low egfp expression, respectively. (C) Embryo at the 20 ss; the bracket with an asterisk in C2 shows area of mixed high- and low-expressing egfp-positive endothelial cells. (D) Embryo at 24 hpf. (E) Confocal micrograph of live Tg(fli1a:dsredex)^um13;(tp1:egfp)^um14 embryo at 7 days postfertilization showing Notch activation (green) and endothelial cells (red); DA, dorsal aorta; ISA, intersomitic artery; ISVe, intersomitic vein. Scale bars: 100 μm (A,C,D); 50 μm (B,E).

Expression Data

Genes:	DsRedx EGFP fli1
Fish:	um14Tg um13Tg; um14Tg
Anatomical Terms:	dorsal aorta dorsal aorta endothelial cell endothelial cell intersegmental artery intersegmental vein intersegmental vessel endothelial cell vascular cord endothelial cell
Stage Range:	14-19 somites to Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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