FIGURE

Fig. 1

ID

ZDB-FIG-140530-3

Publication

Blanco-Sánchez et al., 2014 - Complexes of Usher proteins preassemble at the endoplasmic reticulum and are required for trafficking and ER homeostasis

Other Figures

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Fig. 1

Zebrafish cdh23, ush1c, myo7aa and ift88 mutants have mechanoreceptor structural defects. (A–E) Confocal projections of anterior maculae labeled with phalloidin in (A) wild-type (WT) sibling, (B) cdh23^tj264a, (C) ush1c^fh293, (D) ift88^tz288b and (E) myo7aa^ty220d mutants. (F–H) Confocal optical sections of double immunolabeling of (F) Harmonin (green) and Ift88 (red), (G) Harmonin (green) and Cdh23 (red), and (H) Harmonin (green) and Myo7aa (red). Individual hair cells are shown. (I) Quantification of total number of hair bundles. ne6 analyzed anterior maculae. (J) Quantification of total number of hair cells. ne5 analyzed anterior maculae. (K) Quantification of hair cell death in mutants and wild-type siblings. Number: average number of TUNEL-positive hair cells per macula. ne16 analyzed anterior maculae. Student’s t-test: **P<0.01, *P<0.05. NS, non significant. Black lines represent ± s.e.m. Scale bars: 5 µm.

Expression Data

Genes:	ift88 ush1c
Antibodies:	Ab1-cdh23 Ab1-ift88 Ab2-myo7a
Fish:	WT
Anatomical Terms:	hair cell hair cell kinocilium
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cdh23^tj264/tj264 ift88^tz288/tz288 myo7aa^ty220/ty220 ush1c^fh293/fh293
Observed In:	anterior macula stereocilium bundle hair cell anterior macula hair cell anterior macula apoptotic process hair cell anterior macula epithelium hair cell anterior macula stereocilium
Stage:	Day 5

Phenotype Detail

Acknowledgments

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