Fig. 3

Morpholino mediated knock-down of hapln1a recapitulates all cx43-dependent phenotypes.

(Top row) Total regenerate length was measured in MO and MM treated fins. Hapln1a knock-down resulted in reduced fin length (*). The dotted line represents the amputation plane. (Middle row) Segment length was measured in MO and MM treated fins. Hapln1a knock-down resulted in reduction of segment length (*). Arrowheads identify joints. (Bottom row) Total number of H3P positive cells (red) was counted. Images of representative whole fins stained for H3P are shown for each condition. Knock-down of Hapln1a resulted in reduced cell proliferation (*) compared to the mismatch control. MO represents targeting morpholino; ATG MO targets translation initiation; Splice MO targets splicing event; MM represents control morpholino with 5 mismatches to target sequence; CS represents uninjected/untreated side. All experiments were completed in triplicate with a minimum of five fins treated with MO per trial. Statistical significance was determined by student′s t-test where p<0.05. By student′s t-test, there is no statistical difference between MM and CS. Error bars represent the standard deviation. Scale bar is 50 μm.